NDLI: STRUCTURAL EFFECTS OF VERAPAMIL ON CELL MEMBRANES AND MOLECULAR MODELS

Content Provider	Scientific Electronic Library Online (SciELO)
Author	SUWALSKY, MARIO MUÑOZ, MAURICIO MENNICKENT, SIGRID SOTOMAYOR, CARLOS P BOLOGNIN, SILVIA ZATTA, PAOLO
Abstract	Verapamil is one of the frequently prescribed calcium channel blockers used in the treatment of hypertension and angina pectoris. Results of evaluations of the therapy have led to reports of toxic effects. This study presents several evidences that verapamil affects human cells. Scanning electrón microscopy observations of intact human erythrocytes indicated that they underwent morphological alterations as increasing verapamil concentrations starting from 5 µM changed their discoid normal shape, and finally to hemolysis. Fluorescence spectroscopy on isolated unsealed human erythrocyte membranes confirmed these outcomes. In fact, the assays showed that verapamil induced a significant increase of the anisotropy parameters and a modérate one of the generalized polarization, indicative of enhanced order at the acyl chain and polar head regions of the erythrocyte membrane lipid bilayer. X-ray diffraction experiments on dimyristoylphosphatidylcholine and dimyristoylphosphatidylethanolamine bilayers, classes of the major phospholipids present in both outer and inner sides of the erythrocyte membrane, respectively showed that verapamil perturbed the polar head and acyl chain regions of both lipid bilayers. These interactions were found to be stronger with DMPC bilayers. On the other hand, human SH-SY5Y neuroblastoma cells incubated with verapamil suffered a sharp decrease of cell viability.
Related Links	https://scielo.conicyt.cl/scielo.php?script=sci_abstract&pid=S0717-97072010000100002&lng=en&nrm=iso https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-97072010000100002&lng=en&nrm=iso
Starting Page	1
Ending Page	4
Page Count	4
File Format	PDF
ISSN	07179707
DOI	10.4067/S0717-97072010000100002
Journal	Journal of the Chilean Chemical Society
Issue Number	1
Volume Number	55
Language	English
Publisher	Sociedad Chilena de Química
Publisher Date	2010-01-01
Publisher Institution	University of Concepcion Faculty of Chemical Sciences University of Concepcion Faculty of Pharmacy Universidad Católica de Valparaíso Instituto de Química University of Padova CNR-Centre of Metalloproteins
Publisher Place	Chile Italy
Access Restriction	Open
Content Type	Text
Resource Type	Article
Subject	Chemistry

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