NDLI: In situ quantification and evaluation of ClO−/H2S homeostasis in inflammatory gastric tissue by applying a rationally designed dual-response fluorescence probe featuring a novel H+-activated mechanism

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In situ quantification and evaluation of ClO⁻/H₂S homeostasis in inflammatory gastric tissue by applying a rationally designed dual-response fluorescence probe featuring a novel H⁺-activated mechanism

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In situ quantification and evaluation of ClO⁻/H₂S homeostasis in inflammatory gastric tissue by applying a rationally designed dual-response fluorescence probe featuring a novel H⁺-activated mechanism

Content Provider	Royal Society of Chemistry (RSC)
Author	Cao, Ziping Zhao, Xianen Yu, Fabiao You, Jinmao Chen, Guang Chen, Lingxin Liu, Yuxia Fu, Qiang Li, Guoliang Wang, Hua
Copyright Year	2017
Abstract	Homeostasis of ClO−/H2S plays a crucial role in the damage and repair of gastric tissue, but has rarely been investigated due to the challenge of in situ analysis in the highly acidic gastric environment. Herein, we designed a new H+-activated optical mechanism, involving controllable photoinduced electron transfer (PET) and switch of electron push–pull (SEPP), to develop the simple yet multifunctional probe (Z)-4-(2-benzylidenehydrazinyl)-7-nitrobenzo[c][1,2,5]oxadiazole (BNBD). First, the BNBD probe (Off) was protonated by the highly acidic media to trigger strong fluorescence (On). Then, the analytes ClO− and H2S reacted with the protonated BNBD, leading to ultrasensitive (ClO−: 2.7 nM and H2S: 6.9 nM) fluorescence quenching via the rapid oxidation of CN (50 s) and nitro reduction (10 s), respectively. With the logical discrimination by absorbance/colour (ClO−: 300 nm/colorless and H2S: 400 nm/orange), a strategy for the in situ quantification of ClO−/H2S in gastric mucosa and juice was developed. For the first time, the in situ quantitative monitoring of endogenous H2S and ClO−/H2S homeostasis as well as the pathologic manifestation in gastric mucosa were realized, thus overcoming the challenge of ClO−/H2S analysis under highly acidic conditions and enabling the in situ tissue quantification of ClO−/H2S. In combination with the assessment of mucosal damage, this study confirms the injurious/rehabilitative effects of ClO−/H2S on gastric mucosa (at 50–90 μm depth), which may facilitate the auxiliary diagnosis of stomach diseases induced by oxidative stress.
Starting Page	1619
Ending Page	1627
Page Count	9
File Format	HTM / HTML PDF
ISSN	00032654
Volume Number	142
Issue Number	9
Journal	The Analyst
DOI	10.1039/c7an00244k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Hydrogen sulfide Oxidative stress Hypochlorite Gastric mucosa Photoinduced electron transfer Micrometre Homeostasis Fluorescence Positron emission tomography Push\u2013pull train
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry Electrochemistry

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In situ quantification and evaluation of ClO⁻/H₂S homeostasis in inflammatory gastric tissue by applying a rationally designed dual-response fluorescence probe featuring a novel H⁺-activated mechanism