NDLI: Magnetically-enabled biomarker extraction and delivery system: towards integrated ASSURED diagnostic tools

Content Provider	Royal Society of Chemistry (RSC)
Author	Bauer, Westley S. Gibson, Lauren E. Adams, Nicholas M. Wright, David W. Kimmel, Danielle W. Richardson, Kelly A. Matakala, Hellen K. Conrad, Joseph A. Scherr, Thomas F. Haselton, Frederick R.
Copyright Year	2017
Abstract	Diagnosis of asymptomatic malaria poses a great challenge to global disease elimination efforts. Healthcare infrastructure in rural settings cannot support existing state-of-the-art tools necessary to diagnose asymptomatic malaria infections. Instead, lateral flow immunoassays (LFAs) are widely used as a diagnostic tool in malaria endemic areas. While LFAs are simple and easy to use, they are unable to detect low levels of parasite infection. We have developed a field deployable Magnetically-enabled Biomarker Extraction And Delivery System (mBEADS) that significantly improves limits of detection for several commercially available LFAs. Integration of mBEADS with leading commercial Plasmodium falciparum malaria LFAs improves detection limits to encompass an estimated 95% of the disease reservoir. This user-centered mBEADS platform makes significant improvements to a previously cumbersome malaria biomarker enrichment strategy by improving reagent stability, decreasing the processing time 10-fold, and reducing the assay cost 10-fold. The resulting mBEADS process adds just three minutes and less than $0.25 to the total cost of a single LFA, thus balancing sensitivity and practicality to align with the World Health Organization's ASSURED criteria for point-of-care (POC) testing.
Starting Page	1569
Ending Page	1580
Page Count	12
File Format	HTM / HTML PDF
ISSN	00032654
Volume Number	142
Issue Number	9
Journal	The Analyst
DOI	10.1039/c7an00278e
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Plasmodium Biomarker World Health Organization Malaria Endemism LFA Healthcare Parasitism Reagent Natural reservoir
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry Electrochemistry

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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