NDLI: Investigation of the interactions between methylene blue and intramolecular G-quadruplexes: an explicit distinction in electrochemical behavior

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Investigation of the interactions between methylene blue and intramolecular G-quadruplexes: an explicit distinction in electrochemical behavior

Content Provider	Royal Society of Chemistry (RSC)
Author	Cai, Liang-Yuan Buurma, Niklaas J. Zhang, Xin-Xiang Zhang, Fang-Ting Cao, Ting Zhou, Ying-Lin
Copyright Year	2017
Abstract	G-quadruplex sequences exist in eukaryotic organisms and prokaryotes, and the investigation of the interactions between G-quadruplexes and small molecule ligands is important for gene therapy, biosensor fabrication, fluorescence imaging and so on. Here, we investigated the behaviour of methylene blue (MB), an electroactive molecule, in the presence of different intramolecular G-quadruplexes by an electrochemical method using a miniaturized electrochemical device based on its intrinsic electrochemical properties. Although the effects of MB on different intramolecular G-quadruplex structures are not obvious by circular dichroism spectroscopy, distinct differences in the binding affinities of MB with different intramolecular G-quadruplexes were quickly and easily observed by an electrochemical technique. At the same time, for the human telomerase G-rich sequence (HT), the diffusion current of MB changed sensitively under different ionic conditions due to the formation of different conformations of HT, which indicated that our electrochemical method has the potential to study the influence of metal ions on the conformations of the G-quadruplexes with simplicity, rapid response and low cost. From all these, a new stacking mechanism and rule were obtained, which were also validated by docking studies and isothermal titration calorimetry (ITC).
Starting Page	987
Ending Page	993
Page Count	7
File Format	HTM / HTML PDF
ISSN	00032654
Volume Number	142
Issue Number	6
Journal	The Analyst
DOI	10.1039/c7an00083a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Spectroscopy G-quadruplex Eukaryote Fluorescence Methylene blue Circular dichroism Biosensor Electrochemistry ITC Isothermal titration calorimetry Gene therapy Diffusion Telomerase
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry Electrochemistry

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