NDLI: Characterization of peptide attachment on silicon nanowires by X-ray photoelectron spectroscopy and mass spectrometry

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The Analyst Vol - 142 Issue - 5

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Characterization of peptide attachment on silicon nanowires by X-ray photoelectron spectroscopy and mass spectrometry

Content Provider	Royal Society of Chemistry (RSC)
Author	Dupré, Mathieu Cantel, Sonia Kurylo, Ievgen Szunerits, Sabine Boukherroub, Rabah Enjalbal, Christine Coffinier, Yannick Drobecq, Hervé Melnyk, Oleg
Copyright Year	2017
Abstract	In this paper, we report an original method to immobilize a model peptide on silicon nanowires (SiNWs) via a photolinker attached to the SiNWs’ surface. The silicon nanowires were fabricated by a metal assisted chemical etching (MACE) method. Then, direct characterization of the peptide immobilization on SiNWs was performed either by X-ray photoelectron spectroscopy (XPS) or by laser-desorption/ionization mass spectrometry (LDI-MS). XPS allowed us to follow the peptide immobilization and its photorelease by recording the variation of the signal intensities of the different elements present on the SiNW surface. Mass spectrometry was performed without the use of an organic matrix and peptide ions were produced via a photocleavage mechanism. Indeed, thanks to direct photorelease achieved upon laser irradiation, a recorded predictable peak related to the molecular peptide ion has been detected, allowing the identification of the model peptide. Additional MS/MS experiments confirmed the photodissociation site and confirmed the N-terminal immobilization of the peptide on SiNWs.
Starting Page	969
Ending Page	978
Page Count	10
File Format	HTM / HTML PDF
ISSN	00032654
Volume Number	142
Issue Number	6
Journal	The Analyst
DOI	10.1039/c6an02588a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Chemical milling N-terminus MACE Ionization Laser Extracellular matrix Nanowire battery Photoemission spectroscopy Photodissociation Peptide Mass spectrometry X-ray photoelectron spectroscopy
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry Electrochemistry

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Characterization of peptide attachment on silicon nanowires by X-ray photoelectron spectroscopy and mass spectrometry

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