NDLI: A paper-based platform for detection of viral RNA

Please wait, while we are loading the content...

Quantification of the sources and composition of particulate matter by field-deployable mass spectrometry: implications for air quality and public health

Recent advances in real-time and in situ analysis of an electrode–electrolyte interface by mass spectrometry

Quantitative glycomics using liquid phase separations coupled to mass spectrometry

Application of nanocomposite-based sorbents in microextraction techniques: a review

Mid-infrared fiber-optic evanescent field spectroscopy for in situ monitoring of tetrahydrofuran hydrate formation and dissociation

Doubly charged trimeric cluster ions: effective in mutual chiral recognition of tadalafil and three proton pump inhibitors

Hemispherical platinum : silver core : shell nanoparticles for miRNA detection

Liquid identification by using a micro-electro-mechanical interdigital transducer

PALMA, an improved algorithm for DOSY signal processing

An ultrasensitive electrochemical immunosensor based on the synergistic effect of quaternary Cu₂SnZnS₄ NCs and cyclodextrin-functionalized graphene

An open sandwich immunoassay for detection of 13(R,S)-hydroxy-9(E),11(E)-octadecadienoic acid

A sub-picomolar assay for protein by using cubic Cu₂O nanocages loaded with Au nanoparticles as robust redox probes and efficient non-enzymatic electrocatalysts

Specific and sensitive detection of Plasmodium falciparum lactate dehydrogenase by DNA-scaffolded silver nanoclusters combined with an aptamer

Detection of glycosylation and iron-binding protein modifications using Raman spectroscopy

A paper-based platform for detection of viral RNA

Back cover

The Analyst Vol - 142 Issue - 4

The Analyst Vol - 142 Issue - 3

The Analyst Vol - 142 Issue - 2

A paper-based platform for detection of viral RNA

Content Provider	Royal Society of Chemistry (RSC)
Author	Broyles, David Deo, Sapna K. Dikici, Emre Hunt, Eric A. Zhang, Daohong Daunert, Sylvia
Copyright Year	2017
Abstract	Viral detection presents a host of challenges for even the most sensitive analytical techniques, and the complexity of common detection platforms typically preclude portability. With these considerations in mind, we designed a paper microzone plate-based virus detection system for the detection of viral genetic material that can be performed with simple instruments. The sensing system can detect viral cDNA reverse-transcribed from total RNA extraction by utilizing a biotinylated capture probe and an Alexa Fluor® 647-labeled reporter probe. The biotinylated capture probe was linked to the paper surface via NeutrAvidin® that was physically adsorbed on the paper. After addition of reverse-transcribed sample and reporter probe in sequence, the reverse-transcribed target captured the reporter probe and tethered it to the capture probe in a bridged format. Fluorescence intensity was imaged using a Western blot imaging system, and higher target concentration was visible by the increased emission intensity from Alexa Fluor® 647. By utilizing paper, this detection setup could also serve as a sample concentration method via evaporation, which could remarkably lower the detection limit if needed. This detection platform used Epstein-Barr virus (EBV) RNA as a proof-of-concept by sensing cDNA resulting from reverse transcription and can be further expanded as a general method for other pathogens. EBV is a well-known human tumor virus, which has also recently been linked to the development of cervical cancer. The assay was accomplished within two hours including the room-temperature RNA extraction and reverse transcription steps. Also, this paper microzone plate-based platform can potentially be applicable for the development of point-of-care (POC) detection kits or devices due to its robust design, convenient interface, and easy portability. The experiment could be stopped after each step, and continued at a later time. The shelf-life of the modified paper plate setup was at least 3 months without a discernible change in signal, and the result from day 1 could be read at 3 months – both of which are important criteria for POC analytical testing tools, especially in resource-poor settings. All of the required assay steps could potentially be performed without any significant equipment using inexpensive paper microzone plates, which will be ideal for further development of POC testing devices. Although, this platform is not at the stage where it can be directly used in a point-of-care setting, it does have fundamental characteristics such as a stable platform, a simple detection method, and relatively common reagents that align closely with a POC system.
Starting Page	815
Ending Page	823
Page Count	9
File Format	HTM / HTML PDF
ISSN	00032654
Volume Number	142
Issue Number	5
Journal	The Analyst
DOI	10.1039/c6an02452a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Western blot Epstein\u2013Barr virus Reverse transcriptase RNA Alexa Fluor Virus List of reagents RNA extraction Reporter gene Complementary DNA EBV Emission intensity Cervical cancer
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry Electrochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Quantitative analysis of Epstein-Barr virus (EBV)-related gene expression in patients with chronic active EBV infection.

• LIVER CANCER • Epstein-Barr virus in hepatocellular carcinogenesis

Relationship between Epstein-Barr Virus (EBV) Infection and Viral Load in Immunosuppressive Patients

Seasonal influenza a virus in feces of hospitalized adults technical appendix.

Enhanced Fluorescence Images for Labeled Cells on Silver Island Films

Expression of miRNA-146a in nasopharyngeal carcinoma is upregulated by Epstein-Barr virus latent membrane protein 1.

Persistent epstein-barr virus infection in the common marmoset (callithrix jacchus (1996).

Epstein-Barr virus (EBV) deletions as biomarkers of response to treatment of chronic active EBV.

2002. Regulation of epstein-barr virus lytic cycle activation in malignant and nonmalignant.

A paper-based platform for detection of viral RNA

Similar Documents

Quantitative analysis of Epstein-Barr virus (EBV)-related gene expression in patients with chronic active EBV infection.

• LIVER CANCER • Epstein-Barr virus in hepatocellular carcinogenesis

Relationship between Epstein-Barr Virus (EBV) Infection and Viral Load in Immunosuppressive Patients

Seasonal influenza a virus in feces of hospitalized adults technical appendix.

Enhanced Fluorescence Images for Labeled Cells on Silver Island Films

Expression of miRNA-146a in nasopharyngeal carcinoma is upregulated by Epstein-Barr virus latent membrane protein 1.

Persistent epstein-barr virus infection in the common marmoset (callithrix jacchus (1996).

Epstein-Barr virus (EBV) deletions as biomarkers of response to treatment of chronic active EBV.

2002. Regulation of epstein-barr virus lytic cycle activation in malignant and nonmalignant.

A paper-based platform for detection of viral RNA