NDLI: Detection of glycosylation and iron-binding protein modifications using Raman spectroscopy

Content Provider	Royal Society of Chemistry (RSC)
Author	Correa, Elon Brewster, Victoria L. Goodacre, Royston Ashton, Lorna
Copyright Year	2017
Abstract	In this study we demonstrate the use of Raman spectroscopy to determine protein modifications as a result of glycosylation and iron binding. Most proteins undergo some modifications after translation which can directly affect protein function. Identifying these modifications is particularly important in the production of biotherapeutic agents as they can affect stability, immunogenicity and pharmacokinetics. However, post-translational modifications can often be difficult to detect with regard to the subtle structural changes they induce in proteins. From their Raman spectra apo-and holo-forms of iron-binding proteins, transferrin and ferritin, could be readily distinguished and variations in spectral features as a result of structural changes could also be determined. In particular, differences in solvent exposure of aromatic amino acids residues could be identified between the open and closed forms of the iron-binding proteins. Protein modifications as a result of glycosylation can be even more difficult to identify. Through the application of the chemometric techniques of principal component analysis and partial least squares regression variations in Raman spectral features as a result of glycosylation induced structural modifications could be identified. These were then used to distinguish between glycosylated and non-glycosylated transferrin and to measure the relative concentrations of the glycoprotein within a mixture of the native non-glycosylated protein.
Starting Page	808
Ending Page	814
Page Count	7
File Format	HTM / HTML PDF
ISSN	00032654
Volume Number	142
Issue Number	5
Journal	The Analyst
DOI	10.1039/c6an02516a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Transferrin Ferritin Glycoprotein Glycosylation Regression analysis Raman spectroscopy Protein Biopharmaceutical Immunogenicity Pharmacokinetics Iron-binding proteins Solvent Principal component analysis
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry Electrochemistry

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