NDLI: Quantitative analysis of Epstein-Barr virus (EBV)-related gene expression in patients with chronic active EBV infection.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Iwata, Seiko Wada, Kaoru Tobita, Satomi Gotoh, Kensei Ito, Yoshinori Demachi-Okamura, Ayako Shimizu, Norio Nishiyama, Yukihiro Kimura, Hiroshi
Description	Country affiliation: Japan Author Affiliation: Iwata S ( Department of Virology, Nagoya University Graduate School of Medicine, Nagoya, Japan.)
Abstract	Chronic active Epstein-Barr virus (CAEBV) infection is a systemic Epstein-Barr virus (EBV)-positive lymphoproliferative disorder characterized by persistent or recurrent infectious mononucleosis-like symptoms in patients with no known immunodeficiency. The detailed pathogenesis of the disease is unknown and no standard treatment regimen has been developed. EBV gene expression was analysed in peripheral blood samples collected from 24 patients with CAEBV infection. The expression levels of six latent and two lytic EBV genes were quantified by real-time RT-PCR. EBV-encoded small RNA 1 and BamHI-A rightward transcripts were abundantly detected in all patients, and latent membrane protein (LMP) 2 was observed in most patients. EBV nuclear antigen (EBNA) 1 and LMP1 were detected less frequently and were expressed at lower levels. EBNA2 and the two lytic genes were not detected in any of the patients. The pattern of latent gene expression was determined to be latency type II. EBNA1 was detected more frequently and at higher levels in the clinically active patients. Quantifying EBV gene expression is useful in clarifying the pathogenesis of CAEBV infection and may provide information regarding a patient's disease prognosis, as well as possible therapeutic interventions.
File Format	HTM / HTML
ISSN	00221317
Issue Number	Pt 1
Volume Number	91
e-ISSN	14652099
Journal	Journal of General Virology
Language	English
Publisher	Microbiology Society
Publisher Date	2010-01-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Virology Humans Rna, Messenger Child, Preschool Male Gene Expression Profiling Biosynthesis Leukocytes, Mononuclear Rna, Viral Reverse Transcriptase Polymerase Chain Reaction Herpesvirus 4, Human Journal Article Virology Young Adult Gene Expression Regulation, Viral Adolescent Adult Female Genetics Transcription, Genetic Physiology Epstein-barr Virus Infections Child
Content Type	Text
Resource Type	Article
Subject	Virology

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