NDLI: Thermal source Fourier transform infrared microtomography applied to Arctic sea ice diatoms

Please wait, while we are loading the content...

High-throughput screening of triplex DNA binders from complicated samples by 96-well pate format in conjunction with peak area-fading UHPLC-Orbitrap MS

Correction: Photoinduced electron transfer as a design concept for luminescent redox indicators

Back cover

The Analyst Vol - 142 Issue - 3

The Analyst Vol - 142 Issue - 2

Thermal source Fourier transform infrared microtomography applied to Arctic sea ice diatoms

Content Provider	Royal Society of Chemistry (RSC)
Author	Findlay, Catherine Mundy, C. J. Hirschmugl, Carol J. Morrison, Jason Sedlmair, Julia Gough, Kathleen M.
Copyright Year	2017
Abstract	We have used thermal source Fourier Transform Infrared (FTIR) microtomographic imaging to compare sea ice diatoms growing under different light conditions. A prototype tomography accessory was designed to have sufficient degrees of freedom to align any tilted cylindrical sample relative to the axis of rotation, minimizing the off-axis path traced during rotation. The lightweight device rests on the motorized stage to position the sample in the field-of-view and enable mosaic imaging. Reconstruction routines were tested with simulated and real phantoms, to assess limitations in the Radon back-projection method employed. The distribution and abundance of biochemicals is analysed for targets larger than a single FPA tile. Two and three dimensional (2D and 3D) FTIR spectrochemical images were obtained with a Focal Plane Array (FPA, nominal 1.1 μm pixel edges) for phantoms (polystyrene beads in polyvinyl alcohol matrix) and diatom cells harvested from land fast, first-year ice sites in Resolute Passage (74 43.628′N; 95 33.330′W) and Dease Strait (69° 1.11′N; 105° 21.29′W), Nunavut, Canada. The analysis of relative concentrations of organic matter within the encapsulating silica frustules of diatoms is important for a better understanding of both the physiological state and the individual cellular response to environmental pressures. Analysis of 3D FTIR images of Nitzschia frigida collected from beneath high (17–19 cm) and low (3–7 cm) snow depth revealed higher concentrations of lipids in diatoms collected under low snow cover, uniquely based on spectroscopically determined total 3D cell volume and biochemical content.
Starting Page	660
Ending Page	669
Page Count	10
File Format	HTM / HTML PDF
ISSN	00032654
Volume Number	142
Issue Number	4
Journal	The Analyst
DOI	10.1039/c6an02056a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Radon Diatom Polystyrene Silicon dioxide Frustule Three-dimensional space Canada Nunavut Nitzschia FPA Reconstruction Era Micrometre Polyvinyl alcohol Dease Strait Pixel Sea ice
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry Electrochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Sea ice diatoms and ice edge planktonic diatoms at the northern limit of the.

Hydrocarbon biodegradation by Arctic sea-ice and sub-ice microbial communities during microcosm experiments, Northwest Passage (Nunavut, Canada).

Lipids of the antarctic sea ice diatom Nitzschia cylindrus

All about the marine/sea ice diatom Nitzschia lecointei

Hydrological controls on the diatom assemblage of a seasonal arctic river: Boothia Peninsula, Nunavut, Canada

Characterization of high arctic stream diatom assemblages from Cornwallis Island, Nunavut, Canada

Fram Strait spring ice export and September Arctic sea ice

Metabolic engineering of TiO₂ nanoparticles in Nitzschia palea to form diatom nanotubes: an ingredient for solar cells to produce electricity and biofuel

The role of Fram Strait winter cyclones on sea ice flux and on Spitsbergen air temperatures

Thermal source Fourier transform infrared microtomography applied to Arctic sea ice diatoms

Similar Documents

Sea ice diatoms and ice edge planktonic diatoms at the northern limit of the.

Hydrocarbon biodegradation by Arctic sea-ice and sub-ice microbial communities during microcosm experiments, Northwest Passage (Nunavut, Canada).

Lipids of the antarctic sea ice diatom Nitzschia cylindrus

All about the marine/sea ice diatom Nitzschia lecointei

Hydrological controls on the diatom assemblage of a seasonal arctic river: Boothia Peninsula, Nunavut, Canada

Characterization of high arctic stream diatom assemblages from Cornwallis Island, Nunavut, Canada

Fram Strait spring ice export and September Arctic sea ice

Metabolic engineering of TiO2 nanoparticles in Nitzschia palea to form diatom nanotubes: an ingredient for solar cells to produce electricity and biofuel

The role of Fram Strait winter cyclones on sea ice flux and on Spitsbergen air temperatures

Thermal source Fourier transform infrared microtomography applied to Arctic sea ice diatoms

Metabolic engineering of TiO₂ nanoparticles in Nitzschia palea to form diatom nanotubes: an ingredient for solar cells to produce electricity and biofuel