NDLI: Spatially resolved microfluidic stimulation of lymphoid tissue ex vivo

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High-throughput screening of triplex DNA binders from complicated samples by 96-well pate format in conjunction with peak area-fading UHPLC-Orbitrap MS

Correction: Photoinduced electron transfer as a design concept for luminescent redox indicators

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The Analyst Vol - 142 Issue - 3

The Analyst Vol - 142 Issue - 2

Spatially resolved microfluidic stimulation of lymphoid tissue ex vivo

Content Provider	Royal Society of Chemistry (RSC)
Author	Belanger, Maura C. Ross, Ashley E. Pompano, Rebecca R. Woodroof, Jacob F.
Copyright Year	2017
Abstract	The lymph node is a structurally complex organ of the immune system, whose dynamic cellular arrangements are thought to control much of human health. Currently, no methods exist to precisely stimulate substructures within the lymph node or analyze local stimulus-response behaviors, making it difficult to rationally design therapies for inflammatory disease. Here we describe a novel integration of live lymph node slices with a microfluidic system for local stimulation. Slices maintained the cellular organization of the lymph node while making its core experimentally accessible. The 3-layer polydimethylsiloxane device consisted of a perfusion chamber stacked atop stimulation ports fed by underlying microfluidic channels. Fluorescent dextrans similar in size to common proteins, 40 and 70 kDa, were delivered to live lymph node slices with 284 ± 9 μm and 202 ± 15 μm spatial resolution, respectively, after 5 s, which is sufficient to target functional zones of the lymph node. The spread and quantity of stimulation were controlled by varying the flow rates of delivery; these were predictable using a computational model of isotropic diffusion and convection through the tissue. Delivery to two separate regions simultaneously was demonstrated, to mimic complex intercellular signaling. Delivery of a model therapeutic, glucose-conjugated albumin, to specific regions of the lymph node indicated that retention of the drug was greater in the B-cell zone than in the T-cell zone. Together, this work provides a novel platform, the lymph node slice-on-a-chip, to target and study local events in the lymph node and to inform the development of new immunotherapeutics.
Starting Page	649
Ending Page	659
Page Count	11
File Format	HTM / HTML PDF
ISSN	00032654
Volume Number	142
Issue Number	4
Journal	The Analyst
DOI	10.1039/c6an02042a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Isotropy B cell Lymph node Perfusion T cell Micrometre Albumin Diffusion Polydimethylsiloxane Convection Immune system
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry Electrochemistry

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