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Temporal evolution of cell focal adhesions: experimental observations and shear stress profiles

Content Provider	Royal Society of Chemistry (RSC)
Author	Bershadsky, A. D. Aroush, D. Raz-Ben Zaidel-Bar, R. Wagner, H. D.
Copyright Year	2008
Abstract	Live cells create adhering contacts with substrates viafocal adhesion (FA) sites associated with the termini of actin stress fibers. These FA ‘anchors’ enable cells to adhere firmly and locally to a substrate, and to generate traction that enables cell body displacement. Using time-lapse video microscopy, we have monitored the spontaneous anisotropic changes in FA size and shape, resulting from molecular reorganization of the adhesion sites in live, non-motile rat embryonic fibroblasts adhering to fibronectin-coated glass surfaces. The resulting experimental data on FA growth, saturation and decay is compared with predictions from a number of biophysical models. We find that the growth and saturation regimes for all FAs exhibit a consistent, recurring pattern, whereas the disassembly regime exhibits erratic behavior. We observe that the maximum size of FA areas depends on the FA growth rate: larger FA areas are formed as the growth rate increases. Using a composite mechanics model by which the evolution of the shear stress profile along the FA region can be calculated, we suggest that FA saturation is triggered either by the reaching of a minimum shear stress threshold at the FA back edge, or of a maximum difference between the maximum and minimum shear stress along the FA site.
Starting Page	2410
Ending Page	2417
Page Count	8
File Format	HTM / HTML PDF
ISSN	1744683X
Volume Number	4
Issue Number	12
Journal	Soft Matter
DOI	10.1039/b804643n
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Microscopy Actin Anisotropy Time-lapse photography Shear stress
Content Type	Text
Resource Type	Article
Subject	Chemistry Condensed Matter Physics

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Temporal evolution of cell focal adhesions: experimental observations and shear stress profiles

Theory of the mechanical response of focal adhesions to shear flow.

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