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Back cover

Soft Matter Vol - 7 Issue - 7

Soft Matter Vol - 7 Issue - 6

Soft Matter Vol - 7 Issue - 5

Soft Matter Vol - 7 Issue - 4

Soft Matter Vol - 7 Issue - 3

Tracing nucleation pathways in protein aggregation by using small angle scattering methods

Content Provider	Royal Society of Chemistry (RSC)
Author	Bellissent-Funel, Marie-Claire Vogtt, Karsten Javid, Nadeem Sefcik, Jan Alvarez, Eva
Copyright Year	2011
Abstract	Heat and alcohol-induced nucleation pathways of the whey protein β-lactoglobulin were investigated using small angle neutron and X-ray scattering for structural characterization. Protein solutions at various concentrations were either heated stepwise to 80 °C or mixed with ethanol to 50% (v/v). Heating induces dissociation of the β-lactoglobulin dimer in neutral pH aqueous medium, leading to nucleation at about 75 °C of tetrameric, cylindrical clusters, as indicated by three dimensional rigid body and bead modelling performed to fit scattering curves. In contrast to heating, ethanol addition induces the formation of fairly compact, internally disordered sphere-like clusters composed of rod-like submolecular structural units. At higher concentrations these clusters show typical colloidal behaviour, exhibiting long-range repulsive interactions, as also confirmed by dynamic light scattering measurements. The results contrast the effect of different unfolding scenarios on preferred nucleation pathways in subsequent protein assembly processes in various solution environments.
Starting Page	3906
Ending Page	3914
Page Count	9
File Format	HTM / HTML PDF
ISSN	1744683X
Volume Number	7
Issue Number	8
Journal	Soft Matter
DOI	10.1039/c0sm00978d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Nucleation Rigid body Ethanol Dynamic light scattering X-ray scattering techniques Dissociative Whey protein Protein dimer Protein Tire
Content Type	Text
Resource Type	Article
Subject	Chemistry Condensed Matter Physics

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Use of dynamic light scattering and small-angle X-ray scattering to characterize new surfactants in solution conditions for membrane-protein crystallization.

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Grazing-incidence small-angle X-ray scattering (GISAXS) on small periodic targets using large beams

Tracing nucleation pathways in protein aggregation by using small angle scattering methods

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