NDLI: Nonlinear viscoelasticity of adherent cells is controlled by cytoskeletal tension

Content Provider	Royal Society of Chemistry (RSC)
Author	Kollmannsberger, Philip Fabry, Ben Mierke, Claudia Tanja
Copyright Year	2011
Abstract	The viscoelastic response of living cells to small external forces and deformations is characterized by a weak power law in time. The elastic modulus of cells and the power law exponent with which the elastic stresses decay depend on the active contractile prestress in the cytoskeleton. It is unknown whether this also holds in the physiologically relevant regime of large external forces and deformations. We used magnetic tweezers to apply stepwise increasing forces to magnetic beads bound to the cytoskeleton of different cell lines, and recorded the resulting cell deformation (creep response). The creep response followed a weak power law at all force levels. Stiffness and power law exponent increased with force in all cells, indicating simultaneous stress stiffening and fluidization of the cytoskeleton. The amount of stress stiffening and fluidization differed greatly between cell types but scaled with the contractile prestress as the only free parameter. Our results demonstrate that by modulating the internal mechanical tension, cells can actively control their mechanical properties over an exceedingly large range. This behavior is of fundamental importance for protection against damage caused by large external forces, and allows the cells to adapt to the highly variable and nonlinear mechanical properties of the extracellular matrix.
Starting Page	3127
Ending Page	3132
Page Count	6
File Format	HTM / HTML PDF
ISSN	1744683X
Volume Number	7
Issue Number	7
Journal	Soft Matter
DOI	10.1039/c0sm00833h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Viscoelasticity Elastic modulus Cytoskeleton Extracellular matrix Stiffness Fluidization Power law Dislocation creep
Content Type	Text
Resource Type	Article
Subject	Chemistry Condensed Matter Physics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Nonlinear viscoelasticity of adherent cells is controlled by cytoskeletal tension

Temperature dependent stiffness and visco-elastic behaviour of lipid coated microbubbles using atomic force microscopy

Shear thickening of F-actin networks crosslinked with non-muscle myosin IIB

Measuring the elastic modulus of microgels using microdrops

Power-Law Creep from Discrete Dislocation Dynamics

The mechanics and affine–nonaffine transition in polydisperse semiflexible networks

Subtle variations in polymer chemistry modulate substrate stiffness and fibronectin activity

Structure and mechanical response of protein hydrogels reinforced by block copolymer self-assembly

Soft biological materials and their impact on cell function

Nonlinear viscoelasticity of adherent cells is controlled by cytoskeletal tension

Similar Documents

Nonlinear viscoelasticity of adherent cells is controlled by cytoskeletal tension

Temperature dependent stiffness and visco-elastic behaviour of lipid coated microbubbles using atomic force microscopy

Shear thickening of F-actin networks crosslinked with non-muscle myosin IIB

Measuring the elastic modulus of microgels using microdrops

Power-Law Creep from Discrete Dislocation Dynamics

The mechanics and affine–nonaffine transition in polydisperse semiflexible networks

Subtle variations in polymer chemistry modulate substrate stiffness and fibronectin activity

Structure and mechanical response of protein hydrogels reinforced by block copolymer self-assembly

Soft biological materials and their impact on cell function

Nonlinear viscoelasticity of adherent cells is controlled by cytoskeletal tension