NDLI: LC-MS-based metabolomics reveals metabolic signatures related to glioma stem-like cell self-renewal and differentiation

Content Provider	Royal Society of Chemistry (RSC)
Author	Hu, Peishan Zang, Qingce Zhang, Ruiping Yue, Xiaofei Qiang, Boqin Zhou, Zhi Xu, Xiaoyu Han, Wei Xu, Jing Abliz, Zeper Zhang, Rui Peng, Xiaozhong Chen, Yanhua Li, Shanshan
Copyright Year	2017
Abstract	Gliomas are the most common and lethal primary malignant brain tumors. Recent studies implicate an important role for a rare population of glioma stem cells (GSCs) in glioma maintenance and recurrence. New therapeutic strategies are desperately needed requiring insights into the biological and molecular mechanisms underlying the self-renewal and differentiation of GSCs. We now investigate the metabolic signatures of three glioma cell lines with different stemness using a liquid chromatography-mass spectrometry (LC-MS)-based metabolomics approach. Cellular metabolites differentially expressed in U87MG stem-like cells (SLCs) relative to U87 malignant glioma cells (GCs) and U87MG stem-like cell differentiation cells (SLCDCs) were identified. The specific and significant alterations including nucleotide metabolism, glycerophospholipid metabolism, glutathione metabolism, carnitine metabolism and tryptophan metabolism were characterized. Cell function assays were further used to evaluate the self-renewal ability of SLCs treated with differential metabolites, indicating that these metabolites are involved in the maintenance of stemness. The results provide valuable information on the association of the significantly altered metabolites and metabolic pathways with SLC self-renewal and differentiation.
Starting Page	24221
Ending Page	24232
Page Count	12
File Format	HTM / HTML PDF
ISSN	20462069
Volume Number	7
Issue Number	39
Journal	RSC Advances
DOI	10.1039/c7ra03781c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Metabolomics Glioma Glycerophospholipid Liquid chromatography\u2013mass spectrometry Nucleotide Tryptophan Metabolism Glutathione Carnitine
Content Type	Text
Resource Type	Article
Subject	Chemistry Chemical Engineering

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