NDLI: Electrochemical detection of cholesterol based on competitive host–guest recognition using a β-cyclodextrin/poly(N-acetylaniline)/graphene-modified electrode

Content Provider	Royal Society of Chemistry (RSC)
Author	Yang, Long Fan, Shuangmei Li, Can-Peng Zhao, Hui Ran, Xin Zhao, Genfu
Copyright Year	2015
Abstract	A sensitive and selective electrochemical approach for cholesterol sensing based on a competitive host–guest recognition between β-cyclodextrin (β-CD) and a signal probe (methylene blue)/target molecule (cholesterol) using a β-CD/poly(N-acetylaniline)/graphene (β-CD/PNAANI/Gra)-modified electrode was developed. Due to the host–guest interaction, MB molecules can enter into the hydrophobic inner cavity of β-CD, and the β-CD/PNAANI/Gra modified glassy carbon electrode displays a remarkable anodic peak. In the presence of cholesterol, a competitive interaction to β-CD occurs and the MB molecules are displaced by cholesterol. This results in a decreased oxidation peak current of MB as MB is a well known redox probe and hence can be easily detected using the differential pulse voltammetery technique. A linear response range of 1.00 to 50.00 μM for cholesterol with a low detection limit of 0.50 μM (S/N = 3) was obtained by using the indirect method. The proposed method could be successfully utilized to detect cholesterol in serum samples, and may be expanded to analysis of other non-electroactive species. Besides, the host–guest interaction between cholesterol and β-CD was studied by molecular modeling calculations, which revealed that the complexation could reduce the energy of the system and the complex of 2 : 1 host–guest stoichiometry had the lowest ΔE value of −10.45 kcal mol−1. The molecular docking studies suggested that hydrogen bonding, electrostatic interactions, and hydrophobic interactions should be the major driving forces for the formation of the inclusion complex.
Starting Page	64146
Ending Page	64155
Page Count	10
File Format	HTM / HTML PDF
ISSN	20462069
Volume Number	5
Issue Number	79
Journal	RSC Advances
DOI	10.1039/c5ra11420a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Computational chemistry Stoichiometry Electrode Graphene Docking (molecular) Electrochemistry Redox Hydrophobe Carbon Cholesterol Methylene blue
Content Type	Text
Resource Type	Article
Subject	Chemistry Chemical Engineering

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