NDLI: Carrier free co-immobilization of glucoamylase and pullulanase as combi-cross linked enzyme aggregates (combi-CLEAs)

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Carrier free co-immobilization of glucoamylase and pullulanase as combi-cross linked enzyme aggregates (combi-CLEAs)

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RSC Advances Vol - 3 Issue - 6

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Carrier free co-immobilization of glucoamylase and pullulanase as combi-cross linked enzyme aggregates (combi-CLEAs)

Content Provider	Royal Society of Chemistry (RSC)
Author	Pillai, Meena Surnis, Sharvari Ladole, Mayur Nadar, Shamraja Mulla, Mosin Ambarkar, Sneha Nagavekar, Nupur Desai, Shashikant Talekar, Sachin
Copyright Year	2013
Abstract	Herein we report the successful preparation of carrier free co-immobilized form of glucoamylase and pullulanase using combi-CLEAs method from commercial multi-enzyme preparation OPTIMAX® 7525 HP. Glucoamylase and pullulanase were precipitated using ammonium sulfate, followed by cross-linking for 8 h with 2% (v/v) glutaraldehyde to produce insoluble catalytically active co-immobilized form of enzymes. The effects of precipitant type and cross-linking were studied and the biocatalyst was characterized. Scanning electron microscopy analysis showed that co-immobilized form of enzymes was of spherical structure. For starch hydrolytic activity, shift in optimum pH from 5 to 7 and temperature from 60 to 70 °C were observed after co-immobilization of enzymes. After starch hydrolysis reaction in batch mode, 100, 80 and 30% conversions were obtained with co-immobilized enzymes, mixture of separate CLEAs and free enzymes, respectively. Co-immobilization also enhanced the thermal stability and storage stability. After eight reuses with 30 min reaction time, co-immobilized glucoamylase and pullulanase retained 90 and 85% of its initial activity, respectively.
Starting Page	2265
Ending Page	2271
Page Count	7
File Format	HTM / HTML PDF
ISSN	20462069
Volume Number	3
Issue Number	7
Journal	RSC Advances
DOI	10.1039/c2ra22657j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Hydrolysis Ammonium sulfate Glutaraldehyde Enzyme Starch Hewlett-Packard Glucan 1,4-a-glucosidase OPTIMAX Scanning electron microscope Pullulanase
Content Type	Text
Resource Type	Article
Subject	Chemistry Chemical Engineering

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