NDLI: Non-invasive tumor detection in small animals using novel functional Pluronic nanomicelles conjugated with anti-mesothelin antibody

Content Provider	Royal Society of Chemistry (RSC)
Author	Ding, Hong Law, Wing-Chueng Bergey, Earl J. Hu, Rui Roy, Indrajit Prasad, Paras N. Yong, Ken-Tye Erogbogbo, Folarin Wu, Fang Zhao, Weiwei Huang, Kun
Copyright Year	2011
Abstract	In this study QDs were encapsulated in carboxylated PluronicF127 (F127COOH) triblock polymeric micelles and conjugated with anti-mesothelin antibody for the purpose of alleviating potential toxicity, enhancing the stability and improving targeting efficiency of CdTe/ZnS quantum dots (QDs) in tumors. The amphiphilic triblock polymer of F127COOH contains hydrophilic carboxylated poly(ethylene oxide) (PEO) and hydrophobic poly(propylene oxide) (PPO) units. After encapsulating QDs into carboxylated F127 (F127COOH-QD) micelles, the particles were conjugated with anti-mesothelin antibodies to allow targeting of cancerous areas. The size of the monodispersed spherical QD-containing micelles was determined to be ∼120 nm by dynamic light scattering (DLS). The critical micelle concentration (CMC) was estimated to be 4.7 × 10−7 M. In an in vitro study, the anti-methoselin antibody conjugated F127COOH (Me-F127COOH-QD) nanomicelles showed negligible cytotoxicity to pancreatic cancer cells (Panc-1). Confocal microscopy demonstrated that the Me-F127COOH-QD nanomicelles were taken up more efficiently by Panc-1 cells, due to antibody mediated targeting. An in vivo imaging study showed that Me-F127COOH-QD nanomicelles accumulated at the pancreatic tumor site 15 min after intravenous injection. In addition, the low in vivo toxicity of the nanomicellar formulation was evaluated by pathological assays. These results suggest that anti-mesothein antibody conjugated carboxylated F127 nanomicelles may serve as a promising nanoscale platform for early human pancreatic cancer detection and targeted drug delivery.
Starting Page	1813
Ending Page	1822
Page Count	10
File Format	HTM / HTML PDF
ISSN	20403364
Volume Number	3
Issue Number	4
Journal	Nanoscale
DOI	10.1039/c1nr00001b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Pancreatic tumor Antibody Polymer Cytotoxicity Confocal microscopy Drug delivery Hydrophobe Propylene oxide Critical micelle concentration Dynamic light scattering Pancreatic cancer Amphiphile Ethylene oxide Hydrophile
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology

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