NDLI: Fast quantitative ROS detection based on dual-color single rare-earth nanoparticle imaging reveals signaling pathway kinetics in living cells

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Fast quantitative ROS detection based on dual-color single rare-earth nanoparticle imaging reveals signaling pathway kinetics in living cells

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Fast quantitative ROS detection based on dual-color single rare-earth nanoparticle imaging reveals signaling pathway kinetics in living cells

Content Provider	Royal Society of Chemistry (RSC)
Author	Devys, L. Bouzigues, C. I. Alexandrou, A. Ramodiharilafy, R. Gacoin, T. Abdesselem, M.
Copyright Year	2017
Abstract	Reactive oxygen species (ROS), and notably hydrogen peroxide H2O2, are cellular second messengers that are known to control a variety of signaling processes. They can finely regulate the dynamics of signal transduction, cell response and ultimately tissue function. However, there are very few local, quantitative and time-resolved descriptions of their cellular organization at the scale of molecular reactions, due to the lack of efficient sensors. We thus developed a novel nanoprobe-based ROS detection system using the simultaneous imaging of single lanthanide nanoparticles (YAG:Ce and chemically reduced Gd0.6Eu0.4VO4). We reveal that both particle luminescence signals are controlled by their H2O2 local environment. By simultaneously tracking their luminescence, we devised a new approach providing a quantitative (0.5 μM accuracy in the 1–10 μM range) H2O2 measurement with a 500 ms time resolution, surpassing all existing methods by two orders of magnitude, and revealing previously inaccessible molecular events controlling ROS concentration. We used this nanoprobe in living cells to track fast signaling pathways, by measuring the dynamics of H2O2 intracellular concentrations, induced by endothelin-1 (ET-1) stimulation. We thus revealed the mechanisms controlling ROS production, notably the activity modulation of the ROS-producing enzyme NADPH oxidase by fast (<10 s) EGFR transactivation, and measured quantitatively their kinetic parameters through a minimal analytical model. Altogether, these results illustrate how lanthanide nanoparticle-based sensors are a powerful tool to dynamically probe molecular mechanisms shaping the oxidative cell response.
Starting Page	656
Ending Page	665
Page Count	10
File Format	HTM / HTML PDF
ISSN	20403364
Volume Number	9
Issue Number	2
Journal	Nanoscale
DOI	10.1039/c6nr07413h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Signal transduction Reactive oxygen species Lanthanide Hydrogen peroxide Endothelin Enzyme NADPH oxidase Luminescence EGFR Epidermal growth factor receptor
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology

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