NDLI: A novel graphene-based label-free fluorescence ‘turn-on’ nanosensor for selective and sensitive detection of phosphorylated species in biological samples and living cells

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A novel graphene-based label-free fluorescence ‘turn-on’ nanosensor for selective and sensitive detection of phosphorylated species in biological samples and living cells

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Nanoscale Vol - 8 Issue - 7

Nanoscale Vol - 8 Issue - 6

Nanoscale Vol - 8 Issue - 5

A novel graphene-based label-free fluorescence ‘turn-on’ nanosensor for selective and sensitive detection of phosphorylated species in biological samples and living cells

Content Provider	Royal Society of Chemistry (RSC)
Author	Garg, Bhaskar Ke, Yaotang Ling, Yong-Chien
Copyright Year	2016
Abstract	A novel label-free fluorescence ‘turn-on’ nanosensor has been developed for highly selective and sensitive detection of phosphorylated species (Ps) in biological samples and living cells. The design strategy relies on the use of Ti4+-immobilized polydopamine (PDA) coated reduced graphene oxide (rGO@PDA-Ti4+) that serves as an attractive platform to bind riboflavin 5′-monophosphate molecules (FMNs) through ion-pair interactions between phosphate groups and Ti4+. The as-prepared rGO@PDA-Ti4+-FMNs (nanosensor), fluoresce only weakly due to the ineffective Förster resonance energy transfer between the FMNs and rGO@PDA-Ti4+. The experimental findings revealed that the microwave-assisted interaction of the nanosensor with α-, β-casein, ovalbumin, human serum, non-fat milk, egg white, and living cells (all containing Ps) releases FMNs (due to the high formation constant between phosphate groups and Ti4+), leading to an excellent fluorescence ‘turn-on’ response. The fluorescence spectroscopy, confocal microscopy, and MALDI-TOF MS spectrometry were used to detect Ps both qualitatively and quantitatively. Under the optimized conditions, the nanosensor showed a detection limit of ca. 118.5, 28.9, and 54.8 nM for the tryptic digests of α-, β-casein and ovalbumin, respectively. Furthermore, the standard addition method was used as a bench-mark proof for phosphopeptide quantification in egg white samples. We postulate that the present quantitative assay for Ps holds tremendous potential and may pave the way to disease diagnostics in the near future.
Starting Page	4547
Ending Page	4556
Page Count	10
File Format	HTM / HTML PDF
ISSN	20403364
Volume Number	8
Issue Number	8
Journal	Nanoscale
DOI	10.1039/c5nr07261a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Personal digital assistant Förster resonance energy transfer Ovalbumin Spectroscopy Graphene Ionization Phosphate Fluorescence Riboflavin Confocal microscopy Egg white Nanosensor
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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