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Nanoscale Vol - 2 Issue - 4

Nanoscale Vol - 2 Issue - 3

Nanoscale Vol - 2 Issue - 2

Nanoscale Vol -1

Preparation, characterization and application of polyaniline nanospheres to biosensing

Content Provider	Royal Society of Chemistry (RSC)
Author	Malhotra, Bansi Dhar Pandey, Manoj Kumar Kim, Cheol Gi Das, Maumita Datta, Monika Sumana, Gajjala Dhand, Chetna Srivastava, Avanish Kumar
Copyright Year	2010
Abstract	Polyaniline nanospheres (PANI-NS) prepared by morphological transformation of micelle polymerized camphorsulfonic acid (CSA) doped polyaniline nanotubes (PANI-NT) in the presence of ethylene glycol (EG) have been characterized by X-ray diffraction, atomic force microscopy, transmission electron microscopy, scanning electron microscopy, Fourier transform infra-red and UV-Visible spectroscopy. A PANI-NS (60–80 nm) film deposited onto an indium-tin-oxide (ITO) coated glass plate by the solution casting method has been utilized for covalent immobilization of biomolecules (cholesterol oxidase (ChOx)) viaN-ethyl-N′-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) chemistry for fabrication of a cholesterol biosensor. The ChOx/PANI-NS/ITO bioelectrode detects cholesterol in the concentration range of 25 to 500 mg dL−1 with sensitivity of 1.3 × 10−3 mA mg−1 dL and regression coefficient of 0.98. Further, this PANI-NS based bioelectrode shows fast response time (10 s), low Michaelis–Menten constant (2.5 mM) and shelf-life of 12 weeks. The spherical nanostructure observed in the final morphology of the PANI-NS film is attributed to hydrogen bonding interactions between PANI-NT and EG.
Starting Page	747
Ending Page	754
Page Count	8
File Format	HTM / HTML PDF
ISSN	20403364
Volume Number	2
Issue Number	5
Journal	Nanoscale
DOI	10.1039/b9nr00346k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	EDC Carbodiimide Micelle Michaelis Fourier transform Cholesterol oxidase Scanning electron microscope X-ray crystallography Ultraviolet\u2013visible spectroscopy Fourier Michaelis\u2013Menten kinetics NHS Ethylene glycol Linear regression N-Hydroxysuccinimide Nanostructure Polyaniline Cholesterol Chemistry Transmission electron microscopy Atomic-force microscopy Biosensor Covalent bond Hydrogen bond
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology

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