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Nanoscale Vol - 2 Issue - 4

Nanoscale Vol - 2 Issue - 3

Nanoscale Vol - 2 Issue - 2

Nanoscale Vol -1

A hydrogel-based enzyme-loaded polymersome reactor

Content Provider	Royal Society of Chemistry (RSC)
Author	Hoog, Hans-Peter M. De Rowan, Alan E. Cornelissen, Jeroen J. L. M. Nolte, Roeland J. M. Arends, Isabel W. C. E.
Copyright Year	2010
Abstract	In this study we report the immobilization of enzyme-containing polymersomes into a macromolecular hydrogel. Whereas free enzyme shows progressive leakage from the hydrogel in a period of days, leakage of the polymersome-protected enzyme is virtually absent. The preparation of the hydrogel occurs under mild conditions and does not inhibit the activity of the encapsulated enzymes nor does it affect the structure of the polymersomes. The stability of the polymersome hydrogel architecture is demonstrated by the facile recycling of the polymersomes and their use in repeated reaction cycles. A ‘continuous-flow polymersome reactor’ is constructed in which substrate is added to the top of the reactor and product is collected at the bottom. This set-up allows the use of different enzymes and the processing of multiple substrates, as is demonstrated by the conversion of 2-methoxyphenyl acetate to tetraguaiacol in a reactor loaded with polymersome hydrogels containing the enzymes Candida antarctica lipase B (CALB) and glucose oxidase (GOx).
Starting Page	709
Ending Page	716
Page Count	8
File Format	HTM / HTML PDF
ISSN	20403364
Volume Number	2
Issue Number	5
Journal	Nanoscale
DOI	10.1039/b9nr00325h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Candida albicans Glucose oxidase Enzyme Candida Polymersome Lipase Gel
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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