NDLI: Regulating DNA translocation through functionalized soft nanopores

Content Provider	Royal Society of Chemistry (RSC)
Author	Yeh, Li-Hsien Qian, Shizhi Zhang, Mingkan Hsu, Jyh-Ping
Copyright Year	2012
Abstract	Nanopores have emerged as promising next-generation devices for DNA sequencing technology. The two major challenges in such devices are: (i) find an efficient way to raise the DNA capture rate prior to funnelling a nanopore, and (ii) reduce the translocation velocity inside it so that single base resolution can be attained efficiently. To achieve these, a novel soft nanopore comprising a solid-state nanopore and a functionalized soft layer is proposed to regulate the DNA electrokinetic translocation. We show that, in addition to the presence of an electroosmotic flow (EOF), which reduces the DNA translocation velocity, counterion concentration polarization (CP) occurs near the entrance of the nanopore. The latter establishes an enrichment of the counterion concentration field, thereby electrostatically enhancing the capture rate. The dependence of the ionic current on the bulk salt concentration, the soft layer properties, and the length of the nanopore are investigated. We show that if the salt concentration is low, the ionic current depends largely upon the length of the nanopore, and the density of the fixed charge of the soft layer, but not upon its degree of softness. On the other hand, if it is high, ionic current blockade always occurs, regardless of the levels of the other parameters. The proposed soft nanopore is capable of enhancing the performance of DNA translocation while maintaining its basic signature of the ionic current at high salt concentration. The results gathered provide the necessary information for designing devices used in DNA sequencing.
Starting Page	2685
Ending Page	2693
Page Count	9
File Format	HTM / HTML PDF
ISSN	20403364
Volume Number	4
Issue Number	8
Journal	Nanoscale
DOI	10.1039/c2nr30102d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Electro-osmosis Zeta potential Concentration polarization DNA Nanopore Correlation and dependence Counterion DNA sequencing
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology

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