Nanopore detection of DNA molecules in magnesium chloride solutions.

Content Provider	Europe PMC
Author	Zhang, Yin Liu, Lei Sha, Jingjie Ni, Zhonghua Yi, Hong Chen, Yunfei
Copyright Year	2013
Abstract	High translocation speed of a DNA strand through a nanopore is a major bottleneck for nanopore detection of DNA molecules. Here, we choose MgCl2 electrolyte as salt solution to control DNA mobility. Experimental results demonstrate that the duration time for straight state translocation events in 1 M MgCl2 solution is about 1.3 ms which is about three times longer than that for the same DNA in 1 M KCl solution. This is because Mg2+ ions can effectively reduce the surface charge density of the negative DNA strands and then lead to the decrease of the DNA electrophoretic speed. It is also found that the Mg2+ ions can induce the DNA molecules binding together and reduce the probability of straight DNA translocation events. The nanopore with small diameter can break off the bound DNA strands and increase the occurrence probability of straight DNA translocation events.
ISSN	19317573
Journal	Nanoscale Research Letters
Volume Number	8
PubMed Central reference number	PMC3663764
Issue Number	1
PubMed reference number	23688283
e-ISSN	1556276X
DOI	10.1186/1556-276x-8-245
Language	English
Publisher	Springer
Publisher Date	2013-05-20
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright ©2013 Zhang et al.; licensee Springer.
Subject Keyword	Nanopore DNA sequencing Translocation speed
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Condensed Matter Physics Materials Science