NDLI: Changes in protein expression profiles in bovine endometrial epithelial cells exposed to E. coli LPS challenge

Please wait, while we are loading the content...

Integrated discovery of FOXO1–DNA stabilizers from marine natural products to restore chemosensitivity to anti-EGFR-based therapy for metastatic lung cancer

Biochemical effects of venlafaxine on astrocytes as revealed by ¹H NMR-based metabolic profiling

Control of apoptosis by SMAR1

Discovery of new dual binding TNKS inhibitors of Wnt signaling inhibition by pharmacophore modeling, molecular docking and bioassay

Metabolic profiling of a myalgic encephalomyelitis/chronic fatigue syndrome discovery cohort reveals disturbances in fatty acid and lipid metabolism

The functional consequences and prognostic value of dosage sensitivity in ovarian cancer

Changes in protein expression profiles in bovine endometrial epithelial cells exposed to E. coli LPS challenge

Network pharmacology-based virtual screening of natural products from Clerodendrum species for identification of novel anti-cancer therapeutics

The in silico identification of human bile salt export pump (ABCB11) inhibitors associated with cholestatic drug-induced liver injury

Exploration and analysis of drug modes of action through feature integration

Correction: Elucidating time-dependent changes in the urinary metabolome of renal transplant patients by a combined ¹H NMR and GC-MS approach

Back cover

Molecular BioSystems Vol - 13 Issue - 1

Molecular BioSystems Vol -12

Molecular BioSystems Vol -9

Molecular BioSystems Vol -8

Molecular BioSystems Vol -7

Molecular BioSystems Vol -6

Molecular BioSystems Vol -5

Molecular BioSystems Vol -4

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

Changes in protein expression profiles in bovine endometrial epithelial cells exposed to E. coli LPS challenge

Content Provider	Royal Society of Chemistry (RSC)
Author	Charpigny, Gilles Bonizzi, Luigi Roncada, Paola Chanrot, Metasu Humblot, Patrice Guo, Yongzhi Urbani, Andrea Greco, Viviana Piras, Cristian Soggiu, Alessio
Copyright Year	2017
Abstract	E. coli is one of the most frequently involved bacteria in uterine diseases. Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria involved in pathogenic processes leading to post-partum metritis and endometritis in cattle. It also causes inflammation of the endometrium. The increase of cell proliferation by LPS is part of the inflammatory process. The aim of this study was to investigate possible changes in protein expression in relation to the proliferative response of bEECs after challenge with E. coli-LPS. In vitro culture of bEECs was performed from cow genital tracts collected at a slaughterhouse. In passage 5, bEECs from each of 9 cows (3 series of 3 cows) were exposed to 0, 8, and 16 μg ml−1 LPS for 72 h. At time 0 and 72 h later, attached cells/living cells were counted and for each time and LPS dosage, cells were frozen for proteomic analyses. All samples from the 3 series were analyzed by 2-D gel electrophoresis coupled to MALDI-TOF/TOF mass spectrometry. The samples from the first series were subjected to shotgun nLC-MS/MS analysis. From the whole differential proteomics analysis, 38 proteins were differentially expressed (p < 0.05 to p < 0.001) following exposure to LPS. Among them, twenty-eight were found to be up-regulated in the LPS groups in comparison to control groups and ten were down-regulated. Differentially expressed proteins were associated with cell proliferation and apoptosis, transcription, destabilization of cell structure, oxidative stress, regulation of histones, allergy and general cell metabolism pathways. The de-regulations induced by LPS were consistent with the proliferative phenotype and indicated strong alterations of several cell functions. In addition, some of the differentially expressed proteins relates to pathways activated at the time of implantation. The specific changes induced through those signals may have negative consequences for the establishment of pregnancy.
Starting Page	392
Ending Page	405
Page Count	14
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	13
Issue Number	2
Journal	Molecular BioSystems
DOI	10.1039/c6mb00723f
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Oxidative stress Gel electrophoresis Escherichia coli coli Inflammation Nerve tract Metabolism Shotgun sequencing Protein Metritis Phenotype Uterus Ionization Proteomics Lipopolysaccharide Bacteria Gram-negative bacteria Endometritis Mass spectrometry Endometrium Slaughterhouse The Samples Honduran lempira Apoptosis
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Endometrial gene expression in response to lipopolysaccharide between estrous cycle phases and uterine horns in cattle

from pig uterus endometrium during pregnancy

Tunable accessibility of dye-doped liposomes towards gold nanoparticles for fluorescence sensing of lipopolysaccharide

The lipopolysaccharide transport (Lpt) machinery: A nonconventional transporter for lipopolysaccharide assembly at the outer membrane of Gram-negative bacteria

Immunomodulation in the canine endometrium by uteropathogenic Escherichia coli

Gene Networks and Pathways Involved in LPS-Induced Proliferative Response of Bovine Endometrial Epithelial Cells.

LPS-treatment of bovine endometrial epithelial cells causes differential DNA methylation of genes associated with inflammation and endometrial function

Endotoxin (lipopolysaccharide [LPS]) is located at the outer membrane of the Gram-negative bacteria such as Escherichia coli,

Chemical synthesis of bacterial lipoteichoic acids: An insight on its biological significance

Changes in protein expression profiles in bovine endometrial epithelial cells exposed to E. coli LPS challenge

Similar Documents

Endometrial gene expression in response to lipopolysaccharide between estrous cycle phases and uterine horns in cattle

from pig uterus endometrium during pregnancy

Tunable accessibility of dye-doped liposomes towards gold nanoparticles for fluorescence sensing of lipopolysaccharide

The lipopolysaccharide transport (Lpt) machinery: A nonconventional transporter for lipopolysaccharide assembly at the outer membrane of Gram-negative bacteria

Immunomodulation in the canine endometrium by uteropathogenic Escherichia coli

Gene Networks and Pathways Involved in LPS-Induced Proliferative Response of Bovine Endometrial Epithelial Cells.

LPS-treatment of bovine endometrial epithelial cells causes differential DNA methylation of genes associated with inflammation and endometrial function

Endotoxin (lipopolysaccharide [LPS]) is located at the outer membrane of the Gram-negative bacteria such as Escherichia coli,

Chemical synthesis of bacterial lipoteichoic acids: An insight on its biological significance

Changes in protein expression profiles in bovine endometrial epithelial cells exposed to E. coli LPS challenge