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Molecular BioSystems Vol - 13 Issue - 1

Molecular BioSystems Vol -12

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Molecular BioSystems Vol -2

Metabolomics and proteomics technologies to explore the herbal preparation affecting metabolic disorders using high resolution mass spectrometry

Content Provider	Royal Society of Chemistry (RSC)
Author	Zhao, Hongwei Sun, Hui Liu, Liang Zou, Shiyu Zhou, Xiaohang Ma, Chung Wah Zhang, Aihua Liu, Qi Wang, Xijun
Copyright Year	2017
Abstract	An integrative metabolomics and proteomics approach can provide novel insights in the understanding of biological systems. We have integrated proteome and metabolome data sets for a holistic view of the molecular mechanisms in disease. Using quantitative iTRAQ-LC-MS/MS proteomics coupled with UPLC-Q-TOF-HDMS based metabolomics, we determined the protein and metabolite expression changes in the kidney-yang deficiency syndrome (KYDS) rat model and further investigated the intervention effects of the Jinkui Shenqi Pill (JSP). The VIP-plot of the orthogonal PLS-DA (OPLS-DA) was used for discovering the potential biomarkers to clarify the therapeutic mechanisms of JSP in treating KYDS. The results showed that JSP can alleviate the kidney impairment induced by KYDS. Sixty potential biomarkers, including 5-L-glutamyl-taurine, phenylacetaldehyde, 4,6-dihydroxyquinoline, and xanthurenic acid etc., were definitely up- or down-regulated. The regulatory effect of JSP on the disturbed metabolic pathways was proved by the established metabonomic method. Using pathway analyses, we identified the disturbed metabolic pathways such as taurine and hypotaurine metabolism, pyrimidine metabolism, tyrosine metabolism, tryptophan metabolism, histidine metabolism, steroid hormone biosynthesis, etc. Furthermore, using iTRAQ-based quantitative proteomics analysis, seventeen differential proteins were identified and significantly altered by the JSP treatment. These proteins appear to be involved in Wnt, chemokine, PPAR, and MAPK signaling pathways, etc. Functional pathway analysis revealed that most of the proteins were found to play a key role in the regulation of metabolism pathways. Bioinformatics analysis with the IPA software found that these differentially-expressed moleculars had a strong correlation with the α-adrenergic signaling, FGF signaling, etc. Our data indicate that high-throughput metabolomics and proteomics can provide an insight on the herbal preparations affecting the metabolic disorders using high resolution mass spectrometry.
Starting Page	320
Ending Page	329
Page Count	10
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	13
Issue Number	2
Journal	Molecular BioSystems
DOI	10.1039/c6mb00677a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Metabolomics Taurine Fibroblast growth factor Kidney Histidine PPAR Peroxisome proliferator-activated receptor Bioinformatics KYDS Tyrosine Hormone Quantitative proteomics Wnt signaling pathway FGF JavaServer Pages JSP Metabolome Tryptophan Mitogen-activated protein kinase Proteome MAPK Orthogonality Metabolism Protein Syndrome Pyrimidine metabolism Chemokine Isopropyl alcohol Proteomics Hypotaurine Mass spectrometry Phenylacetaldehyde
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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