NDLI: A coupling system of capillary gel electrophoresis with inductively coupled plasma-mass spectrometry for the determination of double stranded DNA fragments

Please wait, while we are loading the content...

A coupling system of capillary gel electrophoresis with inductively coupled plasma-mass spectrometry for the determination of double stranded DNA fragments

Analysis of animal and plant selenometabolites in roots of a selenium accumulator, Brassica rapa var. peruviridis, by speciation

Suppression of ZIP8 expression is a common feature of cadmium-resistant and manganese-resistant RBL-2H3 cells

Selenium metabolism and excretion in mice after injection of ⁸²Se-enriched selenomethionine

d-Histidine and l-histidine attenuate zinc-induced neuronal death in GT1-7 cells

Synthesis of antitumor azolato-bridged dinuclear platinum(ii) complexes with in vivo antitumor efficacy and unique in vitro cytotoxicity profiles

The role of glutathione in the metabolism of diphenylarsinic acid in rats

Elevated amyloid-β plaque deposition in dietary selenium-deficient Tg2576 transgenic mice

AMP-activated protein kinase-mediated glucose transport as a novel target of tributyltin in human embryonic carcinoma cells

Scanning protein analysis of electrofocusing gels using X-ray fluorescence

Evaluation of quantitative probes for weaker Cu(i) binding sites completes a set of four capable of detecting Cu(i) affinities from nanomolar to attomolar

A platinum complex that binds non-covalently to DNA and induces cell death via a different mechanism than cisplatin

Bioeffects of a combination of trace elements on adipocyte biology

The early molecular processes underlying the neurological manifestations of an animal model of Wilson's disease

Comparative proteomics analysis of sodium selenite-induced apoptosis in human prostate cancer cells

Structural and functional investigation into acetyl-coenzyme A synthase and methyltransferase from human pathogen Clostridium difficile

Back cover

Metallomics Vol - 5 Issue - 4

Metallomics Vol - 5 Issue - 3

Metallomics Vol - 5 Issue - 2

A coupling system of capillary gel electrophoresis with inductively coupled plasma-mass spectrometry for the determination of double stranded DNA fragments

Content Provider	Royal Society of Chemistry (RSC)
Author	Chiba, Koichi Fujii, Shin-ichiro Takatsu, Akiko Inagaki, Kazumi Miyashita, Shin-ichi Nagasawa, Keisuke
Copyright Year	2013
Abstract	The coupling system of capillary gel electrophoresis (CGE) and inductively coupled plasma-mass spectrometry (ICP-MS) was newly developed and successfully applied to the double-stranded (ds) DNA quantification. The developed system combines the separation technique for large biomolecules and element selective detection of ICP-MS. This coupling was achieved by using the modified high performance concentric nebulizer (HPCN) with the PTFE tube (HPCN-PT), which can produce the liquid jet by the flow focusing effect. The HPCN-PT effectively nebulizes the highly viscous solution containing gel buffer even at a low flow rate. At a liquid flow rate of 0.010 mL min−1 and a nebulizer gas flow rate of 1 L min−1, the Sauter mean diameter (D3,2) of primary aerosols generated by the HPCN-PT was 3.4 μm, and over 90% (v/v) of the aerosol droplets were less than 10 μm in diameter. The electrophoresis capillary filled with gel buffer was connected to the HPCN-PT via the interface. This interface has two connectors and an electrode that can connect CE and ICP-MS. After the electrophoretic separation at atmospheric pressure, the samples were transferred to the ICP-MS through the interface by applying additional pressure. Fragments of dsDNA, which were commercially available as a ladder marker solution, were successfully separated and analyzed by measuring 31P+ with CGE-ICP-MS, and a linear calibration curve of the phosphorus standard solution (R2 = 0.999) was obtained from 2.7 to 27 mg kg−1. The detection limit (LOD) and absolute detection limit of P were 3.7 μg kg−1 and 0.6 pg (equivalent to 6 pg of DNA), respectively. This absolute detection limit value was equal to the conventional fluorescence determination of DNA.
Starting Page	424
Ending Page	428
Page Count	5
File Format	HTM / HTML PDF
ISSN	17565901
Volume Number	5
Issue Number	5
Journal	Metallomics
DOI	10.1039/c3mt00057e
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Polytetrafluoroethylene Electrode Atmospheric pressure Gel electrophoresis DNA Aerosol Micrometre Fluorescence Electrophoresis Phosphorus Sauter Nebulizer
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Metals and Alloys Biochemistry Biomaterials Biophysics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Aerosol diagnostics and inductively coupled plasma mass spectrometry with demountable concentric nebulizers

Low-pressure helium inductively coupled plasma mass spectrometry: sample aerosol introduction through a capillary interface from a vibrating mesh nebulizer

Rotating disc nebulizer for inductively coupled plasma optical emission spectrometry

Metal speciation using capillary electrophoresis – inductively coupled plasma atomic emission spectrometry and polytetrafluoroethylene capillaries

Bioimaging of metals in brain tissue by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) and metallomics

Determination of neodymium–fulvic acid binding constants by capillary electrophoresis inductively coupled plasma mass spectrometry (CE-ICP-MS)

Capillary electrophoresis of double-stranded DNA fragments using a new fluorescence intercalating dye EvaGreen.

Determination of phosphorus and metals in human brain proteins after isolation by gel electrophoresis by laser ablation inductively coupled plasma source mass spectrometry

Metal imaging in non-denaturating 2D electrophoresis gels by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) for the detection of metalloproteins

A coupling system of capillary gel electrophoresis with inductively coupled plasma-mass spectrometry for the determination of double stranded DNA fragments

Similar Documents

Aerosol diagnostics and inductively coupled plasma mass spectrometry with demountable concentric nebulizers

Low-pressure helium inductively coupled plasma mass spectrometry: sample aerosol introduction through a capillary interface from a vibrating mesh nebulizer

Rotating disc nebulizer for inductively coupled plasma optical emission spectrometry

Metal speciation using capillary electrophoresis – inductively coupled plasma atomic emission spectrometry and polytetrafluoroethylene capillaries

Bioimaging of metals in brain tissue by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) and metallomics

Determination of neodymium–fulvic acid binding constants by capillary electrophoresis inductively coupled plasma mass spectrometry (CE-ICP-MS)

Capillary electrophoresis of double-stranded DNA fragments using a new fluorescence intercalating dye EvaGreen.

Determination of phosphorus and metals in human brain proteins after isolation by gel electrophoresis by laser ablation inductively coupled plasma source mass spectrometry

Metal imaging in non-denaturating 2D electrophoresis gels by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) for the detection of metalloproteins

A coupling system of capillary gel electrophoresis with inductively coupled plasma-mass spectrometry for the determination of double stranded DNA fragments