NDLI: Gene expression profiling of selenophosphate synthetase 2 knockdown in Drosophila melanogaster

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Gene expression profiling of selenophosphate synthetase 2 knockdown in Drosophila melanogaster

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Metallomics Vol - 8 Issue - 2

Gene expression profiling of selenophosphate synthetase 2 knockdown in Drosophila melanogaster

Content Provider	Royal Society of Chemistry (RSC)
Author	Song, Haiyun Liu, Liying Li, Ping Zhang, Yan Li, Gaopeng Chen, Luonan
Copyright Year	2016
Abstract	Selenium (Se) is an important trace element for many organisms and is incorporated into selenoproteins as selenocysteine (Sec). In eukaryotes, selenophosphate synthetase SPS2 is essential for Sec biosynthesis. In recent years, genetic disruptions of both Sec biosynthesis genes and selenoprotein genes have been investigated in different animal models, which provide important clues for understanding the Se metabolism and function in these organisms. However, a systematic study on the knockdown of SPS2 has not been performed in vivo. Herein, we conducted microarray experiments to study the transcriptome of fruit flies with knockdown of SPS2 in larval and adult stages. Several hundred differentially expressed genes were identified in each stage. In spite that the expression levels of other Sec biosynthesis genes and selenoprotein genes were not significantly changed, it is possible that selenoprotein translation might be reduced without impacting the mRNA level. Functional enrichment and network-based analyses revealed that although different sets of differentially expressed genes were obtained in each stage, they were both significantly enriched in the carbohydrate metabolism and redox processes. Furthermore, protein–protein interaction (PPI)-based network clustering analysis implied that several hub genes detected in the top modules, such as Nimrod C1 and regucalcin, could be considered as key regulators that are responsible for the complex responses caused by SPS2 knockdown. Overall, our data provide new insights into the relationship between Se utilization and several fundamental cellular processes as well as diseases.
Starting Page	354
Ending Page	365
Page Count	12
File Format	HTM / HTML PDF
ISSN	17565901
Volume Number	8
Issue Number	3
Journal	Metallomics
DOI	10.1039/c5mt00134j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Trace element Protein\u2013protein interaction Selenoprotein Messenger RNA Transcriptome Selenocysteine Redox Carbohydrate metabolism Selenium Metabolism Microarray Hawker Siddeley Nimrod
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Metals and Alloys Biochemistry Biomaterials Biophysics

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