NDLI: The Expression of Chicken Selenoprotein W, Selenocysteine-synthase (SecS), and Selenophosphate Synthetase-1 (SPS-1) in CHO-K1 Cells

Content Provider	Springer Nature Link
Author	Han, Yan hui Zhang, Zi wei Shao, Cheng Li, Shu Xu, Shi wen Wang, Xiao long
Copyright Year	2012
Abstract	Selenoprotein W (SelW) has been found to be ubiquitously expressed in tissues in vivo and was purified more than 18 years ago. However, little in vitro research has been performed on SelW from birds. To detect the mRNA levels of chicken SelW in cultured cell lines, chicken SelW cDNA was cloned into an expression vector. The chicken SelW expression construct was then transfected into CHO-K1 cells. Using RT-PCR and real-time quantitative reverse transcription PCR, we detected the expression of the chicken SelW mRNA. Moreover, the selenocysteine-synthase (SecS) and selenophosphate synthetase-1 (SPS-1) mRNA levels were analyzed. The expression of SelW was detected in SelW-transfected cells; no expression was observed in control cells. Significant increases in the SelW mRNA levels were obtained in chicken SelW-transfected cells relative to control cells. SecS mRNA levels were significantly increased in chicken SelW transfected cells. No significant difference in the SPS-1 level was observed. Our findings show that chicken SelW could be studied in vitro and that SecS and SPS-1 may have potential roles in SelW biosynthesis.
Starting Page	61
Ending Page	68
Page Count	8
File Format	PDF
ISSN	01634984
Journal	Biological Trace Element Research
Volume Number	148
Issue Number	1
e-ISSN	15590720
Language	English
Publisher	Humana Press Inc
Publisher Date	2012-02-05
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chicken Selenoprotein W Selenocysteine-synthase Selenophosphate synthetase-1 CHO-K1 Nutrition Oncology Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Biochemistry (medical) Endocrinology, Diabetes and Metabolism Clinical Biochemistry Biochemistry Inorganic Chemistry

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