NDLI: Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip

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Lab on a Chip Vol - 17 Issue - 6

Lab on a Chip Vol - 17 Issue - 5

Lab on a Chip Vol - 17 Issue - 4

Lab on a Chip Vol - 17 Issue - 3

Lab on a Chip Vol - 17 Issue - 2

Lab on a Chip Vol - 17 Issue - 1

Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip

Content Provider	Royal Society of Chemistry (RSC)
Author	Chen, Xiangyu Liu, Gang Huang, Qing Tian, Yangchao Zhong, Jie Zhang, Fengqiu Zhai, Zhimin
Copyright Year	2017
Abstract	This paper reports on the synthesis and application of biocompatible and sensitive SERS nanoparticles for the study of uptake of nanoparticles into living cells in a microfluidic chip through surface-enhanced Raman spectroscopy (SERS). The nanoparticles were fabricated as beta-cyclodextrin-coated silver nanoparticles (Ag@CD NPs) modified with para-aminothiophenol (p-ATP) and folic acid (FA) on the surface. The p-ATP molecules act as the Raman reporter while the FA tags have high affinity for folate receptors (FR) that are over-expressed on the surface cancerous cells, so that the nanoparticles can enter the cells and be monitored by the Raman reporter. Therefore, the nanoparticles could be utilized not only as cell invaders due to endocytosis but also as a SERS sensitive probe to monitor the effect of FR-targeted drugs such as dihydroartemisinin (DHA) that induce the population change of FR on the membrane of living cells. As a result, we have successfully demonstrated that we are able to employ the Ag@CD@p-ATP@FA NPs to evaluate the number of NPs entering living cells quantitatively and correspondingly the drug effect on cancer cells in a well-controlled way.
Starting Page	1306
Ending Page	1313
Page Count	8
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	17
Issue Number	7
Journal	Lab on a Chip
DOI	10.1039/c7lc00053g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Dihydroartemisinin Endocytosis Reporter gene Surface-enhanced Raman spectroscopy Biocompatibility Raman FA DHA Folic acid Cancer
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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