NDLI: Micro vapor bubble jet flow for safe and high-rate fluorescence-activated cell sorting

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Lab on a Chip Vol - 17 Issue - 6

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Micro vapor bubble jet flow for safe and high-rate fluorescence-activated cell sorting

Content Provider	Royal Society of Chemistry (RSC)
Author	Liu, Chengxun Blaszkiewicz, Kamil Lagae, Liesbet Majeed, Bivragh Dusa, Alexandra Vercruysse, Dries Wijs, Koen de Tezcan, Deniz Sabuncuoglu Loo, Josine
Copyright Year	2017
Abstract	Safe, high-rate and cost-effective cell sorting is important for clinical cell isolation. However, commercial fluorescence-activated cell sorters (FACS) are expensive and prone to aerosol-induced sample contamination. Here we report a microfluidic cell sorter allowing high rate and fully enclosed cell sorting. The sorter chip consists of an array of micro heating hotspots. Pulsed resistive heating in the hotspots produces numerous micro vapor bubbles with short duration, which gives rise to a rapid jet flow for cell sorting. With this method, we demonstrated high sorting rate comparable to commercial FACS and the significant enrichment of rare cancer cells. This vapor bubble based cell sorting method can be a powerful tool for contamination-free and affordable clinical cell sorting such as circulating tumor cell isolation and cancer cell therapy.
Starting Page	1287
Ending Page	1296
Page Count	10
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	17
Issue Number	7
Journal	Lab on a Chip
DOI	10.1039/c6lc01560c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Flow cytometry Economic bubble Circulating tumor cell Cancer
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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