NDLI: Multi-step microfluidic device for studying cancer metastasis

Content Provider	Royal Society of Chemistry (RSC)
Author	Manimaran, M. Swaminathan, S. Chaw, K. C. Tay, E. H.
Copyright Year	2007
Abstract	This paper describes a multi-step microfluidic device for studying the deformation and extravasation of primary tumor cells. Prior to extravasation, primary tumor cells undergo sequential steps of deformation through the capillaries, before adhering and transmigrating through the endothelial lining and basement membrane. To study this cascade of events, we fabricated a multi-step microfluidic device whose microgaps were coated with Matrigel to mimic the basement membrane. The microchannel was lined with human microvascular endothelial cells (HMECs) to replicate the endothelial lining. Analysis of deformation, biological and migratory capabilities of various tumor cell lines viz. HepG2, HeLa, and MDA-MB 435S were quantified using the fabricated device. After deformation, the cells' viabilities were significantly reduced and their doubling times were simultaneously increased, indicating changes in their biological capability. However, cell deformation did not significantly reduce their cell motility. Cell motility was co-assessed using the cell's migration rate and the overall population's percentage migration under various conditions (no barrier, Matrigel and Matrigel–HMEC). The device was also used to quantify the effects of Matrigel and the endothelial lining on cell migration. Our results suggest that both played an independent role in inhibiting cell extravasation, with the Matrigel significantly slowing down cell movement and the endothelial lining reducing the total number of transmigrated cells.
Starting Page	1041
Ending Page	1047
Page Count	7
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	7
Issue Number	8
Journal	Lab on a Chip
DOI	10.1039/b707399m
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	HeLa HMEC Extravasation Matrigel Capillary Endothelium Basement membrane
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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