NDLI: Controlled cocultures of HeLa cells and human umbilical vein endothelial cells on detachable substrates

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Controlled cocultures of HeLa cells and human umbilical vein endothelial cells on detachable substrates

Content Provider	Royal Society of Chemistry (RSC)
Author	Kaji, Hirokazu Kawashima, Takeaki Yokoi, Takeshi Nishizawa, Matsuhiko
Copyright Year	2009
Abstract	We investigated the interactions between HeLa cells and human umbilical vein endothelial cells (HUVECs) by monitoring their movements in a controllable coculture system. Two complementary, detachable, cell-substrates, one of polystyrene (PS) and the other of poly(dimethylsiloxane) (PDMS), were fabricated by replica molding. Coculturing was started by mechanically assembling two complementary substrates. One substrate was covered with a confluent layer of HeLa cells and its complement covered with confluent HUVECs. Using this coculture system as a tumor/endothelium model, we found that the HeLa cells migrated towards the HUVECs, while, simultaneously, the HUVECs retreated and that both types of cells migrated approximately twice as rapidly (two hundred microns per twenty-four hours) as they did alone. Additionally, when direct contact between the two cell types was prevented, the HUVECs initially migrated towards the HeLa cells and then retreated. The characteristics of the cell movements, i.e. direction and speed, probably are consequences of cell–cell signaling, with such signals possibly important during tumor cell intra- and extravasation.
Starting Page	427
Ending Page	432
Page Count	6
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	9
Issue Number	3
Journal	Lab on a Chip
DOI	10.1039/b812510d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	HeLa Direct Contact Extravasation Polystyrene Umbilical vein Polydimethylsiloxane Endothelium
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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