NDLI: Microfabrication-based modulation of embryonic stem cell differentiation

Please wait, while we are loading the content...

Temperature dependence of viscosity and density of viscous liquids determined from thermal noise spectra of uncalibrated atomic force microscope cantilevers

A simple and efficient approach for calculating permeability coefficients and HETP for rectangular columns

Micro-well arrays for 3D shape control and high resolution analysis of single cells

Back matter

Back cover

Lab on a Chip Vol - 7 Issue - 7

Lab on a Chip Vol - 7 Issue - 6

Lab on a Chip Vol - 7 Issue - 5

Lab on a Chip Vol - 7 Issue - 4

Lab on a Chip Vol - 7 Issue - 3

Lab on a Chip Vol - 7 Issue - 2

Microfabrication-based modulation of embryonic stem cell differentiation

Content Provider	Royal Society of Chemistry (RSC)
Author	Tilles, Arno W. Cho, Cheul H. Parashurama, Natesh Park, Jaesung Li, Yawen Yarmush, Martin L. Toner, Mehmet Berthiaume, François
Copyright Year	2007
Abstract	Embryonic stem (ES) cells form spontaneous aggregates during differentiation, and cell–cell communication in the aggregates plays an important role in differentiation. The development of a controlled differentiation scheme for ES cells has been hindered by the lack of a reliable method to produce uniform aggregate sizes. Conventional techniques, such as hanging drop and suspension cultures, do not allow precise control over size of ES cell aggregates. To surmount this problem, we microfabricated adhesive stencils to make mouse ES (mES) cell aggregates of specific sizes ranging from 100 µm to 500 µm in diameter. With this technique, we studied the effect of the initial aggregate size on ES cell differentiation. After 20 days of induction of differentiation, we analyzed the stem cell populations using gene and protein expression assays as well as biochemical functions. Notably, we found that germ layer differentiation depends on the initial size of the ES cell aggregate. Among the ES cell aggregate sizes tested, the aggregates with 300 µm diameter showed similar differentiation profiles of three germ layers as embryoid bodies made using the “hanging drop” technique. The smaller (100 µm) aggregates showed the increased expression of ectodermal markers compared to the larger (500 µm) aggregates, while the 500 µm aggregates showed the increased expression of mesodermal and endodermal markers compared to the 100 µm aggregates. These results indicate that the initial size of the aggregate is an important factor for ES cell differentiation, and can affect germ layer selection as well as the extent of differentiation.
Starting Page	1018
Ending Page	1028
Page Count	11
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	7
Issue Number	8
Journal	Lab on a Chip
DOI	10.1039/b704739h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Mouse Gene Microfabrication Stem cell Micrometre Embryoid body Germ layer Protein
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Comparison of Germ Cell Gene Expressions in Spontaneous Monolayer versus Embryoid Body Differentiation of Mouse Embryonic Stem Cells toward Germ Cells

Controlling size, shape and homogeneity of embryoid bodies using poly(ethylene glycol) microwells

Bubble-based acoustic micropropulsors: active surfaces and mixers

Spatial and temporal expression pattern of germ layer markers during human embryonic stem cell differentiation in embryoid bodies

Sustained embryoid body formation and culture in a non-laborious three dimensional culture system for human embryonic stem cells.

Differentiation of mouse embryonic stem cells and their hybrids during embryoid body formation

Efficient formation of uniform-sized embryoid bodies using a compartmentalized microchannel device

Single cell epitaxy by acoustic picolitre droplets

Systematic analysis of embryonic stem cell differentiation in hydrodynamic environments with controlled embryoid body size

Microfabrication-based modulation of embryonic stem cell differentiation

Similar Documents

Comparison of Germ Cell Gene Expressions in Spontaneous Monolayer versus Embryoid Body Differentiation of Mouse Embryonic Stem Cells toward Germ Cells

Controlling size, shape and homogeneity of embryoid bodies using poly(ethylene glycol) microwells

Bubble-based acoustic micropropulsors: active surfaces and mixers

Spatial and temporal expression pattern of germ layer markers during human embryonic stem cell differentiation in embryoid bodies

Sustained embryoid body formation and culture in a non-laborious three dimensional culture system for human embryonic stem cells.

Differentiation of mouse embryonic stem cells and their hybrids during embryoid body formation

Efficient formation of uniform-sized embryoid bodies using a compartmentalized microchannel device

Single cell epitaxy by acoustic picolitre droplets

Systematic analysis of embryonic stem cell differentiation in hydrodynamic environments with controlled embryoid body size

Microfabrication-based modulation of embryonic stem cell differentiation