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| Content Provider | Royal Society of Chemistry (RSC) |
|---|---|
| Author | Wierzbicka, Celina Liu, Mingquan Bauer, David Sellergren, Börje Irgum, Knut |
| Copyright Year | 2017 |
| Abstract | We report on the design and characterization of imprinted cationic host polymers for selective trapping of phosphoserine and phosphotyrosine peptides. A series of imidazolium host monomers were synthesized and characterized with respect to binding affinity and stoichiometry of interaction with salts of phenylphosphonic acid. The strongest binders were subsequently used for the preparation of imprinted polymers in the form of crushed monoliths, using Fmoc-phosphotyrosine-ethyl ester or Fmoc-phosphoserine-ethyl ester as templates in combination with a hydrophilic crosslinking monomer. The polymers were compared with respect to binding and its dependence on solvent, and whether charged or uncharged host monomers were used. The recipes were subsequently implemented in the capillary monolith format for evaluation by micro-liquid chromatography in both buffered and organic media. Results from both tested formats reveal that the cationic host polymers displayed enhanced recognition in polar and buffered media, in contrast to neutral urea-based hosts which showed best results in acetonitrile rich mobile phases. |
| Starting Page | 953 |
| Ending Page | 960 |
| Page Count | 8 |
| File Format | HTM / HTML PDF |
| ISSN | 2050750X |
| Volume Number | 5 |
| Issue Number | 5 |
| Journal | Journal of Materials Chemistry B |
| DOI | 10.1039/c6tb02864k |
| Language | English |
| Publisher | Royal Society of Chemistry |
| Access Restriction | Open |
| Subject Keyword | Phosphoserine Stoichiometry Acid The Strongest Ester Hydrophile Monomer Correlation and dependence Solvent Monolith Chromatography Acetonitrile |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Medicine Materials Science Biomedical Engineering |
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