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Journal of Materials Chemistry B Vol - 5 Issue - 4

Journal of Materials Chemistry B Vol - 5 Issue - 3

Journal of Materials Chemistry B Vol - 5 Issue - 2

Journal of Materials Chemistry B Vol -4

Journal of Materials Chemistry B Vol -3

Journal of Materials Chemistry B Vol -2

Journal of Materials Chemistry B Vol -1

Serum-free culturing of human mesenchymal stem cells with immobilized growth factors

Content Provider	Royal Society of Chemistry (RSC)
Author	Kim, Seong Min Mao, Hongli Ueki, Masashi Ito, Yoshihiro
Copyright Year	2017
Abstract	In vitro expansion of human mesenchymal stem cells (hMSCs) using serum-free culture medium is important for basic research and clinical applications. It is known that some growth factors are required for developing a defined medium for hMSC culture. However, growth factors usually show poor stability, short circulating half-life and a rapid rate of cellular internalization when they are in a diffusible state. A potential way to overcome these problems is to immobilize growth factors on materials. Here, three different types of growth factors, basic fibroblast growth factor, transforming growth factor-beta, and platelet-derived growth factor, were co-immobilized on cell culture dish surfaces by photo-reactive gelatin and used for serum-free hMSC cultures. The results showed that the immobilized growth factors supported cell proliferation similarly to the serum-containing medium. More importantly, the immobilization of growth factors significantly improved their thermal stability and efficiently prolonged their shelf life at 4 °C and 37 °C. Furthermore, the immobilized growth factors could be reused at least three times without losing their stimulation effect on cell proliferation. This photo-reactive gelatin-based immobilization of growth factors appears to be a promising method for serum-free hMSC culturing.
Starting Page	928
Ending Page	934
Page Count	7
File Format	HTM / HTML PDF
ISSN	2050750X
Volume Number	5
Issue Number	5
Journal	Journal of Materials Chemistry B
DOI	10.1039/c6tb02867e
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Mesenchymal stem cell Half-life Fibroblast Growth factor Transforming growth factor beta Cell culture Gelatin
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Materials Science Biomedical Engineering

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