NDLI: Phosphopeptide enrichment and fractionation by using Click OEG-CD matrix

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Back matter

Back cover

Analytical Methods Vol - 4 Issue - 4

Analytical Methods Vol - 4 Issue - 3

Analytical Methods Vol - 4 Issue - 2

Analytical Methods Vol -3

Analytical Methods Vol -2

Analytical Methods Vol -1

Phosphopeptide enrichment and fractionation by using Click OEG-CD matrix

Content Provider	Royal Society of Chemistry (RSC)
Author	Zhao, Yanyan Yan, Jingyu Liang, Xinmiao Li, Xiuling Guo, Zhimou
Copyright Year	2012
Abstract	Reversible protein phosphorylation regulates many significant cellular processes. Identification of phosphorylation sites is vital to elucidate their biofunctions. To aid in phosphoproteome characterization, several selective enrichment methods have been developed, including immobilized metal-ion affinity chromatography (IMAC) and titanium dioxide (TiO2). However, the high pH elution step applied in these two methods, which tends to degrade phosphopeptides. In order to improve phosphopeptide enrichment efficiency, a hydrophilic interaction liquid chromatography (HILIC) based material, cyclodextrin (CD) bonded silica (Click OEG-CD), was synthesized in our group and applied to phosphopeptide enrichment and fractionation. Taking tryptic digest of standard protein α-casein as model sample, the performance of Click OEG-CD in phosphopeptide isolation was investigated. It was found that both the acetonitrile and salt concentrations in mobile phase influence the phosphopeptide enrichment selectivity of the matrix. Under optimized enrichment condition, Click OEG-CD has a similar phosphopeptide enrichment selectivity as commercial TiO2. Meanwhile, phosphopeptides with same charges could be fractionated according to hydrophilicity difference on Click OEG-CD under acetonitrile gradient. Different selectivity is proved with Click OEG-CD from conventional SAX. We demonstrate that Click OEG-CD is an efficient matrix in phosphopeptide enrichment and fractionation.
Starting Page	1244
Ending Page	1251
Page Count	8
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	4
Issue Number	5
Journal	Analytical Methods
DOI	10.1039/c2ay05915k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Phosphorylation IMac Protein Chromatography Silicon dioxide Cyclodextrin Affinity chromatography Acetonitrile PH Extracellular matrix Hydrophile Titanium dioxide Simple API for XML
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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