NDLI: Nanohybrid polymer brushes on silica for bioseparation

Content Provider	Royal Society of Chemistry (RSC)
Author	Kamra, Tripta Bagán, Héctor Zhou, Tongchang Jiang, Lingdong Ye, Lei
Copyright Year	2016
Abstract	Boronic acid based affinity materials are of great importance for effective enrichment of biomolecules containing a cis-diol structure, for example glycoproteins. In this work, we developed a new pH- and temperature-responsive boronate affinity material for effective separation of glycoproteins. A nanohybrid material composed of silica cores and flexible polymer brushes, denoted as Si@poly(NIPAm-co-GMA)@APBA, was prepared via surface-initiated atom transfer radical polymerization (SI-ATRP) in combination with Cu(I)-catalyzed azide–alkyne cycloaddition (CuAAC) click reaction. The size, morphology and composition of the obtained nanohybrid were characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), elemental analysis and thermogravimetric analysis (TGA). The density of polymer brushes on the surface of silica nanoparticles was determined to be 0.7 molecules per nm2. The maximum binding capacities of the nanohybrid Si@poly(NIPAm-co-GMA)@APBA for ovalbumin (OVA) and horseradish peroxidase (HRP) were determined to be 87.6 mg g−1 and 22.8 mg g−1, respectively. Glycoprotein binding on the nanohybrid could be controlled by varying the pH of the binding buffer. By increasing the temperature from 20 °C to 35 °C, glycoprotein binding onto the nanohybrid was decreased. This new pH- and temperature-responsive nanohybrid will be useful for a number of biotechnological and biomedical applications, for example, for protein separation and drug delivery.
Starting Page	3247
Ending Page	3256
Page Count	10
File Format	HTM / HTML PDF
ISSN	2050750X
Volume Number	4
Issue Number	19
Journal	Journal of Materials Chemistry B
DOI	10.1039/c6tb00241b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Fourier Carboxylic acid PH Silicon dioxide Polymer Atom-transfer radical-polymerization Cycloaddition Dynamic light scattering Scanning electron microscope Fourier-transform infrared spectroscopy Thermogravimetric analysis Ovalbumin Original video animation Horseradish peroxidase Glycoprotein Protein Drug delivery
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Materials Science Biomedical Engineering

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