NDLI: REDV–polyethyleneimine complexes for selectively enhancing gene delivery in endothelial cells

Please wait, while we are loading the content...

Templated solvothermal synthesis of magnesium silicate hollow nanospheres with ultrahigh specific surface area and their application in high-performance protein adsorption and drug delivery

Magnetically triggered drug release from biocompatible microcapsules for potential cancer therapeutics

Graphene quantum dots as selective fluorescence sensor for the detection of ascorbic acid and acid phosphatase via Cr(vi)/Cr(iii)-modulated redox reaction

Tunable self-assembly of Irinotecan-fatty acid prodrugs with increased cytotoxicity to cancer cells

Shape engineering boosts antibacterial activity of chitosan coated mesoporous silica nanoparticle doped with silver: a mechanistic investigation

Effective motor neuron differentiation of hiPSCs on a patch made of crosslinked monolayer gelatin nanofibers

The synergetic effect of nano-structures and silicon-substitution on the properties of hydroxyapatite scaffolds for bone regeneration

Oligoethylenimine grafted PEGylated poly(aspartic acid) as a macromolecular contrast agent: properties and in vivo studies

Electrodeposition of chitosan based on coordination with metal ions in situ-generated by electrochemical oxidation

A photo-polymerized poly(N_ε-acryloyl l-lysine) hydrogel for 3D culture of MCF-7 breast cancer cells

Simple synthesis of amino acid-functionalized hydrophilic upconversion nanoparticles capped with both carboxyl and amino groups for bimodal imaging

Application of nanodiamonds in Cu(ii)-based rhodamine B probes for NO detection and cell imaging

REDV–polyethyleneimine complexes for selectively enhancing gene delivery in endothelial cells

Real-time monitoring of a controlled drug delivery system in vivo: construction of a near infrared fluorescence monomer conjugated with pH-responsive polymeric micelles

“Stealthy” chitosan/mesoporous silica nanoparticle based complex system for tumor-triggered intracellular drug release

Design of hydrogels for delayed antibody release utilizing hydrophobic association and Diels–Alder chemistry in tandem

Back cover

Journal of Materials Chemistry B Vol - 4 Issue - 9

Journal of Materials Chemistry B Vol - 4 Issue - 8

Journal of Materials Chemistry B Vol - 4 Issue - 7

Journal of Materials Chemistry B Vol - 4 Issue - 6

Journal of Materials Chemistry B Vol - 4 Issue - 5

Journal of Materials Chemistry B Vol -3

Journal of Materials Chemistry B Vol -2

Journal of Materials Chemistry B Vol -1

REDV–polyethyleneimine complexes for selectively enhancing gene delivery in endothelial cells

Content Provider	Royal Society of Chemistry (RSC)
Author	Lv, Juan Zhang, Wencheng Yang, Jing Feng, Yakai Liu, Wen Ren, Xiangkui
Copyright Year	2016
Abstract	Gene therapy provides a new strategy for promoting endothelialization, and rapid endothelialization has attracted increasing attention for inhibiting thrombosis and restenosis in artificial vascular implants. However, the low transfection efficiency and high cytotoxicity of gene delivery systems prevent their in vivo application. In this study, an endothelial cell (EC)-specific gene carrier with relatively high transfection efficiency and low cytotoxicity was prepared successfully. Using bifunctional hydroxylsuccinimide–poly(ethylene glycol)–maleimide (NHS–PEG–MAL) as the linker, an EC-specific REDV peptide was conveniently grafted onto polyethyleneimine-b-poly(lactide-co-3(S)-methyl-morpholine-2,5-dione)-b-polyethyleneimine (PEI–PLMD–PEI). By varying the molar ratios of REDV to PEI, a series of REDV modified copolymers REDV–PEG-g-PEI–PLMD–PEI-g-PEG–REDV (REDV–PPP) were prepared. Then these copolymers were self-assembled into nanoparticles (NPs) as gene carriers. These NPs could easily condense the EGFP-ZNF580 plasmid (pZNF580) to form REDV peptide functionalized NP/pZNF580 complexes with low cytotoxicity. The fluorescence images, Western blot analysis, and quantitative real-time RT-PCR results verified that the effective transfection of REDV peptide functionalized NP/pZNF580 complexes in ECs was comparable with the positive control of PEI (25 000 Da)/pZNF580 complexes. The high transfection efficiency was attributed to the enhanced cell uptake by the REDV peptide and relied on the quantity of the peptide. Furthermore, the rapid migration of the transfected ECs showed the active function of the expressed ZNF580 protein and further demonstrated that the REDV peptide functionalized NP/pZNF580 complexes could improve the transfection of pZNF580 in ECs. These results provided a useful platform to design EC-specific gene carriers and use gene therapy to enhance endothelialization.
Starting Page	3365
Ending Page	3376
Page Count	12
File Format	HTM / HTML PDF
ISSN	2050750X
Volume Number	4
Issue Number	19
Journal	Journal of Materials Chemistry B
DOI	10.1039/c6tb00686h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	EC NHS Gene Gene therapy Thrombosis Restenosis Transfection Cytotoxicity Endothelium Ethylene glycol Maleimide Peptide Plasmid Fluorescence Western blot Reverse transcription polymerase chain reaction Protein
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Materials Science Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Comb-shaped polymer grafted with REDV peptide, PEG and PEI as targeting gene carrier for selective transfection of human endothelial cells

Targeted delivery of microRNA-126 to vascular endothelial cells via REDV peptide modified PEG-trimethyl chitosan

Biodegradable PEI modified complex micelles as gene carriers with tunable gene transfection efficiency for ECs

Mixed micelles obtained by co-assembling comb-like and grafting copolymers as gene carriers for efficient gene delivery and expression in endothelial cells

Polyethyleneimine functionalized polymer microsphere: a novel delivery vector for cells

Star-shaped copolymer grafted PEI and REDV as a gene carrier to improve migration of endothelial cells

3rd generation poly(ethylene imine)s for gene delivery

The proton permeability of self-assembled polymersomes and their neuroprotection by enhancing a neuroprotective peptide across the blood–brain barrier after modification with lactoferrin

Engineered polyethylenimine/graphene oxide nanocomposite for nuclear localized gene delivery

REDV–polyethyleneimine complexes for selectively enhancing gene delivery in endothelial cells

Similar Documents

Comb-shaped polymer grafted with REDV peptide, PEG and PEI as targeting gene carrier for selective transfection of human endothelial cells

Targeted delivery of microRNA-126 to vascular endothelial cells via REDV peptide modified PEG-trimethyl chitosan

Biodegradable PEI modified complex micelles as gene carriers with tunable gene transfection efficiency for ECs

Mixed micelles obtained by co-assembling comb-like and grafting copolymers as gene carriers for efficient gene delivery and expression in endothelial cells

Polyethyleneimine functionalized polymer microsphere: a novel delivery vector for cells

Star-shaped copolymer grafted PEI and REDV as a gene carrier to improve migration of endothelial cells

3rd generation poly(ethylene imine)s for gene delivery

The proton permeability of self-assembled polymersomes and their neuroprotection by enhancing a neuroprotective peptide across the blood–brain barrier after modification with lactoferrin

Engineered polyethylenimine/graphene oxide nanocomposite for nuclear localized gene delivery

REDV–polyethyleneimine complexes for selectively enhancing gene delivery in endothelial cells