NDLI: Reversible PEGylation and Schiff-base linked imidazole modification of polylysine for high-performance gene delivery

Please wait, while we are loading the content...

A delocalizable cationic headgroup together with an oligo-oxyethylene spacer in gemini cationic lipids improves their biological activity as vectors of plasmid DNA

Reversible PEGylation and Schiff-base linked imidazole modification of polylysine for high-performance gene delivery

The enhanced chemotherapeutic effects of doxorubicin loaded PEG coated TiO₂ nanocarriers in an orthotopic breast tumor bearing mouse model

Bioreducible dextran–polyethylenimine conjugates regulate transgene expression distribution in vivo

Light-responsible DNA hydrogel–gold nanoparticle assembly for synergistic cancer therapy

Graphene film-functionalized germanium as a chemically stable, electrically conductive, and biologically active substrate

Combination delivery of Adjudin and Doxorubicin via integrating drug conjugation and nanocarrier approaches for the treatment of drug-resistant cancer cells

An in vitro study on the collective tumor cell migration on nanoroughened poly(dimethylsiloxane) surfaces

Preparation of polymeric particles in CO₂ medium using non-toxic solvents: discussions on the mechanism of particle formation

Fibrous wound dressings encapsulating essential oils as natural antimicrobial agents

A fluorescent light-up platform with “AIE + ESIPT” characteristics for multi-target detection both in solution and on paper strip

Using oxidant susceptibility of thiol stabilized nanoparticles to develop an inflammation triggered drug release system

A cross-linking graphene oxide–polyethyleneimine hybrid film containing ciprofloxacin: one-step preparation, controlled drug release and antibacterial performance

Effects of functional groups on the structure, physicochemical and biological properties of mesoporous bioactive glass scaffolds

Multiwalled carbon nanotube@reduced graphene oxide nanoribbon heterostructure: synthesis, intrinsic peroxidase-like catalytic activity, and its application in colorimetric biosensing

A colorimetric and ratiometric fluorescent probe for ClO⁻ targeting in mitochondria and its application in vivo

Reduction in thrombosis and bacterial adhesion with 7 day implantation of S-nitroso-N-acetylpenicillamine (SNAP)-doped Elast-eon E2As catheters in sheep

Highly flexible heparin-modified chitosan/graphene oxide hybrid hydrogel as a super bilirubin adsorbent with excellent hemocompatibility

Hydroxyapatite coatings with oriented nanoplate arrays: synthesis, formation mechanism and cytocompatibility

Controlled release and corrosion protection by self-assembled colloidal particles electrodeposited onto magnesium alloys

Low-cost photo-responsive nanocarriers by one-step functionalization of flame-made titania agglomerates with l-Lysine

Cellulose nanofiber–titania nanocomposites as potential drug delivery systems for dermal applications

Dual-response for Hg²⁺ and Ag⁺ ions based on biomimetic funnel-shaped alumina nanochannels

Design of multi-functional linear polymers that capture and neutralize a toxic peptide: a comparison with cross-linked nanoparticles

Tunable stellate mesoporous silica nanoparticles for intracellular drug delivery

A new printable and durable N,O-carboxymethyl chitosan–Ca²⁺–polyphosphate complex with morphogenetic activity

Back cover

Journal of Materials Chemistry B Vol - 3 Issue - 7

Journal of Materials Chemistry B Vol - 3 Issue - 6

Journal of Materials Chemistry B Vol - 3 Issue - 5

Journal of Materials Chemistry B Vol -2

Journal of Materials Chemistry B Vol -1

Reversible PEGylation and Schiff-base linked imidazole modification of polylysine for high-performance gene delivery

Content Provider	Royal Society of Chemistry (RSC)
Author	Li, Yongyong Yue, Dong Shi, Donglu Li, Shuo Yi, Qiangying Gu, Zhongwu Cai, Xiaojun
Copyright Year	2015
Abstract	Gene carriers made from polylysine are of interest in relation to gene therapy but suffer from the lack of transfection efficiency due to limited stability and endosomal escape ability. To address this problem, we designed and developed Schiff-base linked imidazole modified polylysine with a reversible-PEGylation catiomer (SL-ImPEG-SS-PLL) for high efficiency gene delivery. The reversible PEGylation was introduced for in vivo circulation, as well as selective PEG detachment to augment the cellular internalization, while introducing Schiff-base linked imidazole residues into polylysine was expected to accelerate the endosomal escape of the DNA payload, as well as facilitate intracellular DNA unpacking and release, thus significantly enhancing gene delivery efficiency. The size alteration of the SL-I15mPEG-SS-PLL/pDNA polyplexes in the presence of 10 mM GSH suggests stimulus-induced PEG detachment under tumor relevant reduction conditions. Acid–base titration assays indicate that imidazole residues confer on polylysine remarkable buffering ability. The agarose gel retardation assay suggests that the Schiff-base linkages provide an increased DNA binding ability to protect DNA against nucleases and timely intracellular DNA unpacking to permit DNA dissociation from polylysine and access to transcriptional machinery. Biological efficacy assessment of this multifunctional carrier, using pEGFP and pGL-3 as reporter genes, indicates comparable to or even higher transfection efficiencies than gold standard PEI and their transfection efficiencies are slightly affected by serum. More importantly, in vivo transfection of pEGFP reveals that GFP expression was found not only in some of the important organs, such as the liver, spleen, kidneys and lungs, but also in the transplanted carcinoma. These experimental results suggest that the reversible PEGylation and Schiff-base linked imidazole modification make SL-ImPEG-SS-PLL a great potential candidate for an effective and biocompatible gene delivery system.
Starting Page	1507
Ending Page	1517
Page Count	11
File Format	HTM / HTML PDF
ISSN	2050750X
Volume Number	3
Issue Number	8
Journal	Journal of Materials Chemistry B
DOI	10.1039/c4tb01724b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Gene Polylysine Gene therapy Transfection Imidazole PEGylation DNA Acid\u2013base titration Agarose Dissociative Reporter gene Gold standard Green fluorescent protein Liver Spleen Carcinoma Biocompatibility
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Materials Science Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Developing a chitosan supported imidazole Schiff-base for high-efficiency gene delivery

Biocompatible polyethylenimine-graft-dextran catiomer for highly efficient gene delivery assisted by a nuclear targeting ligand

Synthesis and evaluation of N-(2,3-dihydroxypropyl)-PEIs as efficient vectors for nucleic acids

Polyethyleneglycol crosslinked N-(2-hydroxyethyl)-polyethylenimine nanoparticles as efficient non-viral vectors for DNA and siRNA delivery in vitro and in vivo

Terminal modification on mPEG-dendritic poly-(l)-lysine cationic diblock copolymer for efficient gene delivery.

Histone-inspired biomimetic polymeric gene vehicles with excellent biocompatibility and enhanced transfection efficacy

Selenocystine-Derived Label-Free Fluorescent Schiff Base Nanocomplex for siRNA Delivery Synergistically Kills Cancer Cells

Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers

Self-assembling Janus dendritic polymer for gene delivery with low cytotoxicity and high gene transfection efficiency

Reversible PEGylation and Schiff-base linked imidazole modification of polylysine for high-performance gene delivery

Similar Documents

Developing a chitosan supported imidazole Schiff-base for high-efficiency gene delivery

Biocompatible polyethylenimine-graft-dextran catiomer for highly efficient gene delivery assisted by a nuclear targeting ligand

Synthesis and evaluation of N-(2,3-dihydroxypropyl)-PEIs as efficient vectors for nucleic acids

Polyethyleneglycol crosslinked N-(2-hydroxyethyl)-polyethylenimine nanoparticles as efficient non-viral vectors for DNA and siRNA delivery in vitro and in vivo

Terminal modification on mPEG-dendritic poly-(l)-lysine cationic diblock copolymer for efficient gene delivery.

Histone-inspired biomimetic polymeric gene vehicles with excellent biocompatibility and enhanced transfection efficacy

Selenocystine-Derived Label-Free Fluorescent Schiff Base Nanocomplex for siRNA Delivery Synergistically Kills Cancer Cells

Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers

Self-assembling Janus dendritic polymer for gene delivery with low cytotoxicity and high gene transfection efficiency

Reversible PEGylation and Schiff-base linked imidazole modification of polylysine for high-performance gene delivery