NDLI: Preparation of protein imprinted polymer beads by Pickering emulsion polymerization

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Back cover

Journal of Materials Chemistry B Vol - 3 Issue - 6

Journal of Materials Chemistry B Vol - 3 Issue - 5

Journal of Materials Chemistry B Vol -2

Journal of Materials Chemistry B Vol -1

Preparation of protein imprinted polymer beads by Pickering emulsion polymerization

Content Provider	Royal Society of Chemistry (RSC)
Author	Bülow, Leif Kamra, Tripta Zhou, Tongchang Zhang, Ka Ye, Lei
Copyright Year	2015
Abstract	We present a new method for preparation of protein-specific polymer beads based on surface molecular imprinting in Pickering emulsion. In the first step, adult human hemoglobin (Hb) was adsorbed on silica nanoparticles. The protein-coated silica particles were then used to stabilize an oil-in-water emulsion (Pickering emulsion) composed of cross-linking monomer in the oil phase. After free radical polymerization of the oil phase, the protein-silica particles were removed to leave Hb-imprinted sites on the polymer surface. The protein-imprinted polymer microspheres were characterized by scanning electron microscopy and their selectivity was investigated by protein binding analysis. The new synthetic method based on Pickering emulsion polymerization produced easily accessible Hb binding sites on the surface of spherical polymer particles, which are useful for protein separation, purification and analysis.
Starting Page	1254
Ending Page	1260
Page Count	7
File Format	HTM / HTML PDF
ISSN	2050750X
Volume Number	3
Issue Number	7
Journal	Journal of Materials Chemistry B
DOI	10.1039/c4tb01605j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Pickering Polymer Molecular imprinting Pickering emulsion Hemoglobin Silicon dioxide Emulsion Monomer Radical polymerization Scanning electron microscope Protein Polymerization
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Materials Science Biomedical Engineering

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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