NDLI: A novel room temperature ionic liquid sol–gel matrix for amperometric biosensor application

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A novel room temperature ionic liquid sol–gel matrix for amperometric biosensor application

Content Provider	Royal Society of Chemistry (RSC)
Author	Shi, Lihong Li, Zhiying Wang, Meijia Li, Jinghong Liu, Yang Liu, Hongtao
Copyright Year	2005
Abstract	A novel type of ionic liquid (IL) sol–gel hybrid material was developed for amperometric biosensor application. This material was prepared by the hydrolysis of tetraethyl orthosilicate in a 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM+BF4−) solution. The viscous IL prevented the cracking of the sol–gel derived glasses. Horseradish peroxidase (HRP) was immobilized in the hybrid material for the production of an amperometric hydrogen peroxide biosensor. The IL sol–gel enzyme electrodes retained the high activity of HRP and provided long-term stability of HRP in storage. The morphology of the matrix-containing enzyme was characterized by scanning electron microscopy. The characteristics of the biosensor were also investigated by cyclic voltammetry and chronoamperometry. In the presence of ferrocene as a mediator, the biosensor exhibited an excellent stability and sensitivity, and the linear calibration ranged from 0.02 to 0.26 mM with a detection of 1.1 µM. The apparent Michaelis–Menten constant of the immobilized HRP was 2.0 mM.
Starting Page	655
Ending Page	658
Page Count	4
File Format	HTM / HTML PDF
ISSN	14639262
Volume Number	7
Issue Number	9
Journal	Green Chemistry
DOI	10.1039/b504689k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	HRP Michaelis Ionic liquid Biosensor Hydrolysis Tetraethyl orthosilicate Butyl group Tetrafluoroborate Horseradish peroxidase Hydrogen peroxide Enzyme Scanning electron microscope Cyclic voltammetry Chronoamperometry Ferrocene Michaelis\u2013Menten kinetics
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Pollution

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