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Sol-gel horseradish peroxidase biosensor for hydrogen peroxide detection by chemiluminescence
| Content Provider | Semantic Scholar |
|---|---|
| Author | Díaz, Aurora Navas Peinado, M. C. Ramos Minguez, Mara |
| Copyright Year | 1998 |
| Abstract | Horseradish peroxidase (HRP) was immobilized by microencapsulation in sol‐gel crystals derived from tetramethyl orthosilicate. A sol‐gel biosensor based on the chemiluminescence reaction of the hydrogen peroxide‐luminol‐HRP system was developed. Calibration plots for hydrogen peroxide have been established by chemiluminescence measurements in a cuvette and through an optical fibre modified at its end with the immobilized HRP gel. The detection limit was 6.710 ˇ4 M and the linear range was 0.1‐3.0 mM. The relative standard deviation was 7.1% (na4). This method was applied to hydrogen peroxide determination in disinfectant solutions for contact lenses. # 1998 Elsevier Science B.V. |
| Starting Page | 221 |
| Ending Page | 227 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/S0003-2670(98)00080-4 |
| Alternate Webpage(s) | http://www.biblioteca.uma.es/bbldoc/articulos/1672480x.pdf |
| Alternate Webpage(s) | https://doi.org/10.1016/S0003-2670%2898%2900080-4 |
| Volume Number | 363 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
