NDLI: Quantification of 4-hydroxy-2-nonenal-protein adducts in the <i>in vivo</i> gastric digesta of mini-pigs using a GC-MS/MS method with accuracy profile validation

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Quantification of 4-hydroxy-2-nonenal-protein adducts in the in vivo gastric digesta of mini-pigs using a GC-MS/MS method with accuracy profile validation

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Quantification of 4-hydroxy-2-nonenal-protein adducts in the in vivo gastric digesta of mini-pigs using a GC-MS/MS method with accuracy profile validation

Content Provider	Royal Society of Chemistry (RSC)
Author	Comte, Blandine Thomas, Agnès Joly, Charlotte Delosière, Mylène Rémond, Didier Laurentie, Michel Pujos-Guillot, Estelle Guy, Alexandre Chantelauze, Céline Gladine, Cécile Santé-Lhoutellier, Véronique Durand, Thierry Dufour, Claire Durand, Denys
Copyright Year	2016
Abstract	Hydroxyalkenals are lipid oxidation end-products resulting from the oxidation of polyunsaturated fatty acids (PUFA). This study aimed at quantifying the production of 4-hydroxy-2-nonenal-protein adducts (HNE-P) via Michael addition from n-6 PUFA oxidation in the gastric digesta of mini-pigs after the consumption of meat-based meals with different plant antioxidant contents. Using the accuracy profile procedure, we validated an extraction protocol for the quantification of HNE-P by GC-MS/MS in gastric contents. The formation of HNE-P in the gastric compartment was observed for the first time, with concentrations ranging from less than 0.52 to 1.33 nmol HNE-P per 500 mg digesta. Nevertheless, most gastric HNE-P levels were below the limit of quantification of 0.52 nmol HNE-P per 500 mg digesta. In this animal study, the protective effect of plant antioxidant sources on HNE-P formation was not evidenced contrasting with the results using TBARS as markers.
Starting Page	3497
Ending Page	3504
Page Count	8
File Format	HTM / HTML PDF
ISSN	20426496
Volume Number	7
Issue Number	8
Journal	Food & Function
DOI	10.1039/c6fo00413j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Michael Lipid peroxidation Polyunsaturated fat Michael reaction Antioxidant Gas chromatography\u2013mass spectrometry Animal testing
Content Type	Text
Resource Type	Article
Subject	Medicine Food Science

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Quantification of 4-hydroxy-2-nonenal-protein adducts in the in vivo gastric digesta of mini-pigs using a GC-MS/MS method with accuracy profile validation

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