NDLI: Separation and purification of an anti-tumor peptide from rapeseed (<i>Brassica campestris</i> L.) and the effect on cell apoptosis

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Separation and purification of an anti-tumor peptide from rapeseed (Brassica campestris L.) and the effect on cell apoptosis

Content Provider	Royal Society of Chemistry (RSC)
Author	Zhang, Jing Yuan, Qiang Shi, Jiayi Ju, Xingrong Wang, Lifeng Xie, Huihui
Copyright Year	2016
Abstract	Rapeseed peptides were prepared by means of the combined methods of the laboratory bacteria enzyme synergy and the solid-state fermentation of rapeseed meal. The rapeseed peptides were separated and purified with the tumor cell in vitro anti-proliferative activity as an index. Moreover, a kind of rapeseed peptide component RSP-4-3-3 (rapeseed anti-tumor peptide RSP-4-3-3) with high activity was selected. Furthermore, by using reversed-phase high performance liquid chromatography (RP-HPLC) coupled with electrospray ionization mass spectrometry (ESI-MS/MS), the analysis result of its possible amino acid sequence showed that it was Trp–Thr–Pro (408.2 Da). Inverted microscope observation technology and western blot experiments were applied to explore the antitumor impact of the rapeseed peptide RSP-4-3-3 on tumor cells. The results showed that the rapeseed antitumor peptide RSP-4-3-3 could significantly change the morphological features of the HepG2 cells in vitro and cause apoptosis, thus inhibiting the proliferation of the HepG2 cells.
Starting Page	2239
Ending Page	2248
Page Count	10
File Format	HTM / HTML PDF
ISSN	20426496
Volume Number	7
Issue Number	5
Journal	Food & Function
DOI	10.1039/c6fo00042h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Trp Rapeseed Bacteria Enzyme Fermentation Peptide Rana Sura Padakkama Reversed-phase chromatography High-performance liquid chromatography Electrospray ionization Ionization Mass spectrometry Acid Inverted microscope Western blot Apoptosis
Content Type	Text
Resource Type	Article
Subject	Medicine Food Science

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