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| Content Provider | Royal Society of Chemistry (RSC) |
|---|---|
| Author | Padilha, Marina Saad, Susana Marta Isay Costa, Mayra Garcia Maia Morales, Martha Lissete Villarreal Vieira, Antonio Diogo Silva |
| Copyright Year | 2016 |
| Abstract | The survival of two probiotic strains – Lactobacillus acidophilus La-5 and Bifidobacterium animalis Bb-12 – incorporated into probiotic (PC) and into synbiotic (SC, with inulin + fructooligosaccharides, respectively, at 7.5 and at 2.5 g per 100 g) petit-suisse cheese was investigated in the beginning (day 1) and at the end (28 days) of storage at 4 °C when the food products were subjected to in vitro gastrointestinal simulated assays. Species-specific quantitative real time PCR (qPCR) combined with propidium monoazide (PMA–qPCR) was employed to quantify the strains. Initial La-5 and Bb-12 populations were always above 7 log CFU g−1. The presence of the prebiotic ingredients in SC improved the Bb-12 and La-5 resistance after the 6 h assay, with higher populations in all the in vitro stages and throughout the storage period (p < 0.05), leading to equal or superior survival rates (SR) in SC of both probiotic strains, in the beginning as well as at the end of storage. The mean La-5 SR were 58% (PC) and 67% (SC), whereas the mean Bb-12 SR were 60% (PC) and 79% (SC). Our findings suggest that the addition of a prebiotic mixture in petit-suisse cheese was advantageous, since it improved both the Bb-12 and La-5 viability and tolerance under in vitro gastrointestinal simulated conditions, both in the fresh product and in the product refrigerated for 28 days. |
| Starting Page | 2312 |
| Ending Page | 2319 |
| Page Count | 8 |
| File Format | HTM / HTML PDF |
| ISSN | 20426496 |
| Volume Number | 7 |
| Issue Number | 5 |
| Journal | Food & Function |
| DOI | 10.1039/c5fo01592h |
| Language | English |
| Publisher | Royal Society of Chemistry |
| Access Restriction | Open |
| Subject Keyword | PMA CFU Probiotic Lactobacillus acidophilus Lavochkin La-5 Bifidobacterium animalis Personal computer Inulin Cheese Polymerase chain reaction Propidium monoazide |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Food Science |
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