NDLI: Metallacarboranes as tunable redox potential electrochemical indicators for screening of gene mutation

Content Provider	Royal Society of Chemistry (RSC)
Author	Teixidor, Francesc Viñas, Clara Pariente, Félix Lorenzo, Encarnación Fuentes, Isabel Zaulet, Adnana García-Mendiola, Tania Bayon-Pizarro, Victoria
Copyright Year	2016
Abstract	The substitution of hydrogen with chlorine in the metallacarborane [3,3-Fe(1,2-closo-C2B9H11)2]− cluster modulates the formal potential of the Fe3+/Fe2+ redox couple, shifting it to a more positive value. Hence, very similar redox probes with a wide range of formal potentials, ranging from negative to positive values, are available. Thus, we have achieved the synthesis and studied the electrochemical behaviour of the sodium salt of [3,3-Fe(8,9,12-Cl3-1,2-closo-C2B9H8)2]− in aqueous media. This strategy allows tuning of the redox potential of the [3,3-Fe(1,2-closo-C2B9H11)2]− framework with a minor change in its shape and dimensions. We also describe the interaction of the prepared [3,3-Fe(8,9,12-Cl3-1,2-closo-C2B9H8)2]− and the pristine [3,3-Fe(1,2-closo-C2B9H11)2]− with DNA. These studies have been carried out not only with DNA in solution but also with DNA immobilized on screen-printed gold electrodes. The results obtained point to a strong interaction between the metallacarboranes and DNA, to a different extent with single stranded DNA (ssDNA) compared to double stranded DNA (dsDNA). This property makes them selective and wide-ranging potential electrochemical indicators of hybridization. The suitability of these new redox indicators for selective DNA biosensor development has been probed by the direct detection of two different mutations associated with cystic fibrosis in PCR amplicons extracted from blood cells.
Starting Page	5786
Ending Page	5797
Page Count	12
File Format	HTM / HTML PDF
ISSN	20416520
Volume Number	7
Issue Number	9
Journal	Chemical Science
DOI	10.1039/c6sc01567k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Polymerase chain reaction Sodium Hydrogen Chlorine DNA Biosensor Fibrosis Redox Electrochemistry Strong interaction
Content Type	Text
Resource Type	Article
Subject	Chemistry

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