NDLI: DNA micelle flares: a study of the basic properties that contribute to enhanced stability and binding affinity in complex biological systems

Content Provider	Royal Society of Chemistry (RSC)
Author	Wu, Cuichen Zhang, Liqin Cui, Cheng Wan, Shuo Cansiz, Sena Sumerlin, Brent S. Chen, Tao Hou, Weijia Cai, Ren Zhang, Xiaobing Liu, Yuan Wang, Yanyue Wu, Yuan Sun, Hao Tan, Weihong
Copyright Year	2016
Abstract	DMFs are spherical DNA–diacyllipid nanostructures formed by hydrophobic effects between lipid tails coupled to single-stranded DNAs. Such properties as high cellular permeability, low critical micelle concentration (CMC) and facile fabrication facilitate intracellular imaging and drug delivery. While the basic properties of NFs have been amply described and tested, few studies have characterized the fundamental properties of DMFs with particular respect to aggregation number, dissociation constant and biostability. Therefore, to further explore their conformational features and enhanced stability in complex biological systems, we herein report a series of characterization studies. Static light scattering (SLS) demonstrated that DMFs possess greater DNA loading capacity when compared to other DNA-based nanostructures. Upon binding to complementary DNA (cDNA), DMFs showed excellent dissociation constants (Kd) and increased melting temperatures, as well as constant CMC (10 nM) independent of DNA length. DMFs also present significantly enhanced stability in aqueous solution with nuclease and cell lysate. These properties make DMFs ideal for versatile applications in bioanalysis and theranostics studies.
Starting Page	6041
Ending Page	6049
Page Count	9
File Format	HTM / HTML PDF
ISSN	20416520
Volume Number	7
Issue Number	9
Journal	Chemical Science
DOI	10.1039/c6sc00066e
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Cell membrane Static light scattering Dissociation constant Drug delivery Hydrophobe Dissociative Lipid Critical micelle concentration Complementary DNA Bioanalysis DNA CMC Aggregation number Nuclease
Content Type	Text
Resource Type	Article
Subject	Chemistry

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DNA micelle flares: a study of the basic properties that contribute to enhanced stability and binding affinity in complex biological systems† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6sc00066e

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DNA micelle flares: a study of the basic properties that contribute to enhanced stability and binding affinity in complex biological systems

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DNA micelle flares: a study of the basic properties that contribute to enhanced stability and binding affinity in complex biological systems