NDLI: In situ electrochemical investigation of the interaction between bacteria Xylella fastidiosa DNA and copper(II) using DNA-electrochemical biosensors

Please wait, while we are loading the content...

In situ electrochemical investigation of the interaction between bacteria Xylella fastidiosa DNA and copper(II) using DNA-electrochemical biosensors

Content Provider	Directory of Open Access Journals (DOAJ)
Author	W.B.S. Machini A.M. Oliveira-Brett
Abstract	New emerging bacterial plant diseases are spreading fast in different geographical regions, and effective safety measures have not been found. Xylella fastidiosa (Xf) is a Gram-negative bacterial plant pathogen classified as a priority quarantine pest, which affects economically important agricultural crops. Although there is no treatment for diseases caused by Xf, copper-based compounds are widely applied to its hosts.A Xf-DNA-electrochemical biosensor based on bacterial DNA from Xf, is reported here for the first time, and the Cu(II)-Xf-dsDNA interaction was investigated in situ. The Cu(II)-DNA interaction was also evaluated using ct-dsDNA-, poly[G]- and poly[A]-electrochemical biosensors. The Cu(II)-Xf-dsDNA interaction occurs by Cu(II) binding at different sites, independent of the bacterial DNA sequence, leading to the condensation/aggregation of Xf-DNA strands due to the formation of a rigid Cu(II)-Xf-dsDNA complex structure. Cu(II) did not cause oxidative DNA damage. Resistance of the bacterial Xf-dsDNA to Cu(II) was observed.
Related Links	http://www.sciencedirect.com/science/article/pii/S138824812100059X
ISSN	13882481
DOI	10.1016/j.elecom.2021.106975
Journal	Electrochemistry Communications
Volume Number	125
Language	English
Publisher	Elsevier
Publisher Date	2021-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Industrial electrochemistry Chemistry Bacterial Dna Dna-electrochemical Biosensor Pesticide-dna Interaction Xylella Fastidiosa
Content Type	Text
Resource Type	Article
Subject	Electrochemistry

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in