NDLI: A unique approach toward near-infrared fluorescent probes for bioimaging with remarkably enhanced contrast

Content Provider	Royal Society of Chemistry (RSC)
Author	Meng, Hong-Min Feng, Suling Mao, Guo-Jiang Su, Li Gong, Yi-Jun Zhang, Xiao-Bing Zhang, Guisheng Tan, Weihong
Copyright Year	2016
Abstract	Near-infrared (NIR) fluorescent probes are attractive molecular tools for bioimaging because of their low autofluorescence interference, deep tissue penetration, and minimal damage to sample. However, most previously reported NIR probes exhibit small Stokes shift, typically less than 30 nm, and low fluorescence quantum yield, strictly limited contrast and spatial resolution for bioimaging. Herein, by expanding the π-conjugated system of rhodamine B, while, at the same time, keeping its rigid and planar structure, we reported an efficient NIR dye, HN7, with large stokes shift of 73 nm and fluorescence quantum yield as high as 0.72 in ethanol, values superior to those of such traditional cyanine NIR dyes as Cy5. Using HN7, living cells, tissues and mice were imaged, and the results showed significantly enhanced contrast, improved spatial resolution, and satisfactory tissue imaging depth when compared to Cy5. Moreover, the nonfluorescent spirocyclic structure of rhodamine B is an inherent component of HN7; therefore, our strategy provided a universal platform for the design of efficient NIR turn-on bioimaging probes for various targets. As a proof-of-concept, two different NIR probes, HN7-N2 and HN7-S for NO and Hg2+, respectively, were designed, synthesized, and successfully applied for the imaging of NO and Hg2+ in living cells, tissues and mice, respectively, demonstrating the potential bioimaging applications of the new probes. In sum, this new type of dye may present new avenues for the development of efficient NIR fluorescent probes for contrast-enhanced imaging in biological applications.
Starting Page	2275
Ending Page	2285
Page Count	11
File Format	HTM / HTML PDF
ISSN	20416520
Volume Number	7
Issue Number	3
Journal	Chemical Science
DOI	10.1039/c5sc04014k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Rhodamine Dye Stokes shift Stokes Ethanol NIR Autofluorescence Fluorescence Cyanine Quantum tunnelling Spiro compound
Content Type	Text
Resource Type	Article
Subject	Chemistry

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