NDLI: Optical control of neuronal activity using a light-operated GIRK channel opener (<b>LOGO</b>)

Content Provider	Royal Society of Chemistry (RSC)
Author	Weaver, C. David Trauner, Dirk Burgstaller, Jessica Isacoff, Ehud Y. Schönberger, Matthias Levitz, Joshua Barber, David M. Baier, Herwig
Copyright Year	2016
Abstract	G-protein coupled inwardly rectifying potassium (GIRK) channels are expressed throughout the human body and are an integral part of inhibitory signal transduction pathways. Upon binding of Gβγ subunits released from G-protein coupled receptors (GPCRs), GIRK channels open and reduce the activity of excitable cells via hyperpolarization. As such, they play a role in cardiac output, the coordination of movement and cognition. Due to their involvement in a multitude of pathways, the precision control of GIRK channels is an important endeavour. Here, we describe the development of the photoswitchable agonist LOGO (the Light-Operated GIRK channel Opener), which activates GIRK channels in the dark and is rapidly deactivated upon exposure to long wavelength UV irradiation. LOGO is the first photochromic K+ channel opener and selectively targets channels that contain the GIRK1 subunit. It can be used to optically silence action potential firing in dissociated hippocampal neurons and LOGO exhibits activity in vivo, controlling the motility of zebrafish larvae in a light-dependent fashion. We envisage that LOGO will be a valuable research tool to dissect the function of GIRK channels from other GPCR dependent signalling pathways.
Starting Page	2347
Ending Page	2352
Page Count	6
File Format	HTM / HTML PDF
ISSN	20416520
Volume Number	7
Issue Number	3
Journal	Chemical Science
DOI	10.1039/c5sc04084a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Agonist Photochromism Channel opener Action potential Zebrafish Wavelength Signal transduction Integral GIRK Membrane potential Cardiac output G protein-coupled inwardly-rectifying potassium channel Potassium channel KCNJ3
Content Type	Text
Resource Type	Article
Subject	Chemistry

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